仔猪BPI基因表达水平与大肠杆菌F18菌株感染的关系

doi:10.3724/SP.J.1005.2011.01225

遗传 ›› 2011, Vol. 33 ›› Issue (11): 1225-1230.doi: 10.3724/SP.J.1005.2011.01225

仔猪BPI基因表达水平与大肠杆菌F18菌株感染的关系

叶兰¹, 訾臣¹, 刘璐¹, 朱璟¹, 谢恺舟¹, 朱国强^{2, 3, 4}, 黄小国^{3, 4}, 包文斌^{1, 3, 4}, 吴圣龙^{1, 3, 4}, 王金玉¹

1. 扬州大学动物科学与技术学院, 江苏省动物遗传繁育与分子设计重点实验室, 扬州 225009 2. 扬州大学兽医学院, 扬州 225009 3. 江苏省现代种猪分子选育工程技术研究中心, 常州 213149 4. 江苏(扬州)规模猪场高效健康养殖公共技术服务中心, 扬州 225009

收稿日期:2011-01-07 修回日期:2011-05-11 出版日期:2011-11-20 发布日期:2011-11-25
通讯作者: 吴圣龙 E-mail:slwu@yzu.edu.cn
基金资助:
转基因生物新品种培育科技重大专项(编号：2009ZX08006-004B), 江苏省科技支撑计划(农业)(编号：BE2008364, BE2009330-2, BE2010371, BE2010450)和常州市农业科技攻关项目(编号：CE20092004, CE20102006)资助

Study on the relationship between the expression of BPI gene and Escherichia coli F18 infection in piglets

YE Lan¹, ZI Chen¹, LIU Lu¹, ZHU Jing¹, XIE Kai-Zhou¹, ZHU Guo-Qian^{2, 3, 4}, HUANG Xiao-Guo^{3, 4}, BAO Wen-Bin^{1, 3, 4}, WU Sheng-Long^{1, 3, 4}, WANG Jin-Yu¹

1. Animal Science and Technology College, Yangzhou University, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, China 2. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China 3. Jiangsu Engineering Research Centre for Molecular Breeding of Breeder Pig, Changzhou 213149, China 4. Jiangsu (Yangzhou) Public Technical Service Centre for Efficient and Healthy Culture Technology in Large-scale Swine Farms, Yangzhou 225009, China

Received:2011-01-07 Revised:2011-05-11 Online:2011-11-20 Published:2011-11-25
Contact: WU Sheng-Long E-mail:slwu@yzu.edu.cn

摘要/Abstract

摘要： 文章利用已建立的苏太猪F18大肠杆菌病抗性和敏感性资源家系群体作为实验材料, 分别选择8头35日龄左右生长性状基本一致的大肠杆菌 F18菌株抗性和敏感性断奶仔猪, 运用Real-time PCR方法检测BPI基因mRNA在断奶仔猪各个组织的分布情况, 并比较其在大肠杆菌 F18菌株抗性型和敏感型断奶仔猪个体间的差异表达水平, 为探讨该基因在免疫和抗大肠杆菌F18菌株感染中的作用提供依据。结果表明, 在对所有个体检测的11个组织中, BPI基因在心、肝、脾、肺、肾、胃、肌肉、胸腺、淋巴结中几乎不表达, 或表达量很低, 但在十二指肠和空肠中表达量很高。在十二指肠和空肠中, BPI基因在抗性组的表达量均显著高于敏感组的表达量(P<0.05)。由此表明, BPI基因对抗断奶仔猪肠道中大肠杆菌F18菌株的感染可能具有直接作用, 并且个体对大肠杆菌F18菌株的抗性可能与BPI基因在肠道中表达量上调有关。

关键词: 大肠杆菌F18菌株, 断奶仔猪, BPI基因, Real-time PCR

Abstract: Based on the established resource populations of Sutai pig, the expression of BPI gene was assayed by Real-time PCR to detect the tissue expression and analyze the differential expression between Escherichia coli F18-resistant and sensitive piglets. This study aimed at providing a theoretical foundation for further research on the role BPI gene in host immunity and resistance to E. coli F18. The results showed that the expression of BPI gene was extremely low or undetectable in tissues including heart, liver, spleen, lung, kidney, stomach, muscle, thymus, and lymph nodes, which was in a stark contrast to the significantly high levels in duodenum and jejunum. In the tissues of both jejunum and duodenum, the mRNA expression of BPI gene in resistant individuals was significant higher than that in the sensitive individuals (P<0.05). The results suggested that BPI gene was likely to be related to the intestinal infection caused by E. coli F18. It is possible that the increased expression of BPI gene in intestinal is in connection with the resistance to E. coli F18.

Key words: Escherichia coli F18, post-weaning piglets, BPI gene, Real-time PCR

叶兰，訾臣，刘璐，朱璟，谢恺舟，朱国强，黄小国，包文斌，吴圣龙，王金玉. 仔猪BPI基因表达水平与大肠杆菌F18菌株感染的关系[J]. 遗传, 2011, 33(11): 1225-1230.

YE Lan, ZI Chen, LIU Lu, ZHU Jing, XIE Kai-Zhou, ZHU Guo-Qian, HUANG Xiao-Guo, BAO Wen-Bin. Study on the relationship between the expression of BPI gene and Escherichia coli F18 infection in piglets[J]. HEREDITAS, 2011, 33(11): 1225-1230.

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仔猪BPI基因表达水平与大肠杆菌F18菌株感染的关系

Study on the relationship between the expression of BPI gene and Escherichia coli F18 infection in piglets

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