遗传 ›› 2011, Vol. 33 ›› Issue (11): 1245-1250.doi: 10.3724/SP.J.1005.2011.01245

• 研究报告 • 上一篇    下一篇

镜鲤体长性状的QTL定位分析

张天奇1,2, 张晓峰2, 谭照君1,2, 曹柱1,2, 王宣朋2,3, 李文升2,3, 储志远1,2, 孙效文2   

  1. 1. 大连海洋大学生命科学与技术学院, 大连116023 2. 中国水产科学研究所黑龙江水产研究及农业部北方鱼类生物工程育种重点开放实验室, 哈尔滨 150070 3. 上海海洋大学水产与生命技术学院, 上海 201306
  • 收稿日期:2010-12-16 修回日期:2011-03-30 出版日期:2011-11-20 发布日期:2011-11-25
  • 通讯作者: 孙效文 E-mail:sunxw2002@163.com
  • 基金资助:

    国家重大基础研究发展规划(973)项目(编号:2010CB126305)资助

Mapping and genetic effect analysis of quantitative trait loci related to body length in mirror carp (Cyprinus carpio L.)

ZHANG Tian-Qi1,2, ZHANG Xiao-Feng2, TAN Zhao-Jun1,2, CAO Zhu1,2, WANG Xuan-Peng2,3, LI Wen-Sheng2,3, CHU Zhi-Yuan1,2, SUN Xiao-Wen2   

  1. 1. College of Life Science and Technology, Dalian Ocean University, Dalian 116023, China 2. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China 3. College of Aqua-life Science and Technology, Shanghai Ocean University, Shanghai 201306, China
  • Received:2010-12-16 Revised:2011-03-30 Online:2011-11-20 Published:2011-11-25
  • Contact: SUN Xiao-Wen E-mail:sunxw2002@163.com

摘要: 以镜鲤良种后代为祖父母本所培育的杂交F2群体的68个个体为材料, 利用553个分子标记(217个SSR和336个SNP标记)对其进行基因型检测, 运用JoinMap4.0软件包对遗传连锁图谱进行构建。利用MapQTL5.0区间作图法(Interval mapping)进行QTL检测, 通过置换实验(1 000次重复)确定连锁群显著性水平阈值。在对体长的区间定位中共检测到12个与体长性状相关的QTLs区间, 分布在BL-1-1(SNP0137-SNP1481)、BL-4-1 (SNP0092- HLJ797)、BL-5-1(SNP1268-HLJ423)、BL-7-1(HLJ870-SNP0702)、BL-12-1(SNP0922-HLJ639)、BL-16-1 (HLJE351- SNP0674)、BL-25-1(SNP0394-SNP0862)、BL-35-1(HLJ668-SNP0832)、BL-43-1(SNP0389-SNP1425)、BL-47-1 (HLJ057-HLJ1113)、BL-47-2(HLJ1439-HLJ1418)等11个连锁群上, 解释表型变异范围是13.8%~64.9%, 其中贡献率大于20%的主效QTLs有8个, 是体长性状的主效QTLs区间。

关键词: 镜鲤, 体长, 数量性状定位, 分子辅助育种

Abstract: A group of F2 hybrids of mirror carp including 68 individuals was used to construct a linkage map using JoinMap4.0 software. Of 560 markers genotyped, 507 markers (143 SSR markers, 33 EST-SSR markers, and 321 SNP markers) were assigned to the map, which comprised of 62 linkage groups. Quantitative traits loci (QTLs) associated with body length were identified by interval mapping of the software MapQTL 5.0. A linkage group wide permutation test (1 000 replicates) determined the significance of the maximum LOD value over the various intervals analyzed for each linkage group. Twelve QTLs were identified for body length on the linkage groups of BL-1-1 (SNP0137-SNP1481), BL-4-1 (SNP0092-HLJ797), BL-5-1 (SNP1268-HLJ423), BL-7-1 (HLJ870-SNP0702), BL-12-1 (SNP0922-HLJ639), BL-16-1 (HLJE351-SNP0674), BL-25-1 (SNP0394-SNP0862), BL-35-1 (HLJ668-SNP0832), BL-43-1 (SNP0389-SNP1425), BL-47-1 (HLJ057-HLJ1113), BL-47-2 (HLJ1439-HLJ14180), which explained 13.8% to 64.9% of the total variation of body length. Eight major QTLs explained over 20% of the phenotypic variation, which were major QTLs associated with the body length of mirror carp.

Key words: mirror carp (Cyprinus carpio L.), body length, quantitative trait locus mapping, molecular maker–assisted selection