镜鲤体长、体高、体厚性状QTL定位分析

doi:10.3724/SP.J.1005.2011.01366

遗传 ›› 2011, Vol. 33 ›› Issue (12): 1366-1373.doi: 10.3724/SP.J.1005.2011.01366

镜鲤体长、体高、体厚性状QTL定位分析

郑先虎^1,2, 匡友谊¹, 鲁翠云^1,2, 王宣朋^1,2, 李文升^1,2, 吕伟华^1,3, 孙效文¹

1. 中国水产科学院黑龙江水产研究所, 农业部淡水水产生物技术与遗传育种实验室, 哈尔滨 150070 2. 上海海洋大学水产与生命学院, 上海 201306 3. 大连海洋大学海洋环境工程学院, 大连 116023

收稿日期:2011-03-03 修回日期:2011-05-03 出版日期:2011-12-20 发布日期:2011-12-25
通讯作者: 孙效文 E-mail:sunxw2002@163.com
基金资助:
公益性行业(农业)科研专项(编号：200903045), 国家重点基础研究发展计划(973计划)项目(编号：2010CB126305)和中央级公益性科研院所基本科研业务费专项(编号：201006)资助

Quantitative trait locus analysis of standard length, body depth and body thickness in mirror carp (Cyprinus carpio L.)

ZHENG Xian-Hu^1,2, KUANG You-Yi¹, LU Cui-Yun^1,2, WANG Xuan-Peng^1,2, LI Wen-Sheng^1,2, LÜ Wei-Hua^1,3, SUN Xiao-Wen¹

1. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Harbin 150070, China 2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China 3. College of Marine Environmental Engineering, Dalian Ocean University, Dalian 116023, China

Received:2011-03-03 Revised:2011-05-03 Online:2011-12-20 Published:2011-12-25

摘要/Abstract

摘要： 以镜鲤全同胞家系为材料, 用246个SSR和306个SNP标记构建了鲤鱼的连锁图谱, 利用GridQTL软件对体长(SL)、体高(H)、体厚(BT)和体长/体高(SLH)进行了QTL定位分析。结果显示：共检测到14个相关的QTL, 分布在7个连锁群上。其中, 7个与体长相关的QTL——LG6、LG17、LG21、LG23和LG35连锁群上的QTL为显著水平(P<0.05), LG1和LG28上达到极显著水平(P<0.01), 可解释表型变异为6.6%~12.6%; 3个与体高相关的QTL均为极显著水平(P<0.01)位于LG17、LG23和LG28上, 可解释表型变异分别为11.6%、12.7%和 15.6%; 2个与体厚相关的QTL均为显著水平(P<0.05)位于LG23和LG28上, 可解释表型变异分别为8.6%和7.2%; 2个与体长/体高相关的QTL均为显著水平(P<0.05)位于LG21和LG35上, 可解释表型变异均为8.2%。

关键词: 鲤, 连锁图谱, QTL, 体长, 体高, 体厚

Abstract: Based on a full-sib family, the genetic linkage map was constructed with 246 microsatellite and 306 SNP markers, which was used to detect the QTLs for standard length (SL), body depth (H), body thickness (BT), and the ratio of standard length and body depth (SLH) in mirror carp by GridQTL software. The results indicated that a total of 14 related QTLs distributed on the 7 linkage groups were obtained. Seven QTLs were related to standard length, of which the linkage groups of LG6, LG17, LG21, LG23, and LG35 were at 5% significant level, and linkage group LG1 and LG28 were at 1% significant level, which explained 6.6%—12.6% of the phenotypic variance. Three QTLs were identified for body depth on the linkage groups of LG17, LG23 and LG28 (P < 0.01), accounting for 11.6%, 12.7%, and 15.6% of the phenotypic variance, respectively. Two QTLs were associated with body thickness on the linkage of LG23 and LG28 (P < 0.05), which explained 8.6% and 7.2% of the phenotypic variation, respectively. Two QTLs were responsible for the ratio of standard length and body depth on the linkage of LG21 and LG35 (P < 0.05), both of which explained 8.2% of the phenotypic variance. The results provide a useful reference for further candidate gene research and molecular marker as-sisted selection in mirror carp.

Key words: mirror carp (Cyprinus carpio L.), genetic linkage map, QTL, standard length, body depth, body thickness

郑先虎，匡友谊，鲁翠云，王宣朋，李文升，吕伟华，孙效文. 镜鲤体长、体高、体厚性状QTL定位分析[J]. 遗传, 2011, 33(12): 1366-1373.

ZHENG Xian-Hu, KUANG You-Yi, LU Cui-Yun, WANG Xuan-Peng, LI Wen-Sheng, Lü Wei-Hua, SUN Xiao-Wen. Quantitative trait locus analysis of standard length, body depth and body thickness in mirror carp (Cyprinus carpio L.)[J]. HEREDITAS, 2011, 33(12): 1366-1373.

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镜鲤体长、体高、体厚性状QTL定位分析

Quantitative trait locus analysis of standard length, body depth and body thickness in mirror carp (Cyprinus carpio L.)

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