遗传 ›› 2012, Vol. 34 ›› Issue (1): 72-78.doi: 10.3724/SP.J.1005.2012.00072

• 研究报告 • 上一篇    下一篇

黄颡鱼MSTN基因多态性及其与生长性状的相关性分析

朱媛媛1,2, 梁宏伟2,3, 李忠2,3, 罗相忠2,3, 李林1,2, 张志伟1,2, 邹桂伟2,3   

  1. 1. 华中农业大学水产学院, 武汉 430070 2. 中国水产科学研究院长江水产研究所, 武汉430223 3. 中国水产科学研究院淡水渔业研究中心, 无锡214081
  • 收稿日期:2011-05-12 修回日期:2011-07-17 出版日期:2012-01-20 发布日期:2012-01-25
  • 通讯作者: 邹桂伟 E-mail:zougw@yfi.ac.cn
  • 基金资助:

    国家高技术研究发展规划项目(863计划)(编号:2011AA100401)和中央级公益性科研院所基本科研业务费专项资金项目资助

Polymorphism of MSTN gene and its association with growth traits in yellow catfish (Pelteobagruse fulvidraco)

ZHU Yuan-Yuan1,2, LIANG Hong-Wei2,3, LI Zhong2,3, LUO Xiang-Zhong2,3, LI Lin1,2, ZHANG Zhi-Wei1,2, ZOU Gui-Wei2,3   

  1. 1. Fisheries College of Huazhong Agricultural University, Wuhan 430070, China 2. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China 3. Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
  • Received:2011-05-12 Revised:2011-07-17 Online:2012-01-20 Published:2012-01-25

摘要: 肌肉生长抑制素基因(Myostatin, MSTN)属于转化生长因子β家族, 其主要功能是负向调控肌肉的生长发育。采用PCR-SSCP技术对黄颡鱼MSTN基因进行单核苷酸多态性检测和分型, 并与其生长性状进行关联分析。结果表明, 在第一内含子部分检测到1个缺失位点和2个突变位点(T1003del、G1022A和T1063G), 基因型分别为:AA、AB、CC、CDDD; 在第三外显子部分检测到1个突变位点(T132C), 基因型分别为:EEEF。关联性分析表明, AA基因型个体的全长、体长、体高、体厚、头长和体重显著大于CDDD基因型(P<0.05), AA基因型雌性个体的全长、体长、体高、体厚、头长、尾柄高、尾柄厚和体重也显著大于DD基因型(P<0.05)。由此推断, AA基因型是影响雌性黄颡鱼生长性状的有利基因型, DD基因型是影响雌性黄颡鱼生长性状的不利基因型, 可以尝试利用这两个位点对雌性黄颡鱼进行标记辅助选育。

关键词: 黄颡鱼, MSTN, 突变位点, 多态性, 生长性状

Abstract: Myostatin (MSTN) gene has negative influence on the growth of muscle, which belongs to transforming growth factor-β family. Using single-strand conformation polymorphism (SSCP), single nucleotide polymorphisms (SNPs) of MSTN gene were detected in yellow catfish. Correlation analysis between MSTN polymorphisms and growth traits was also carried out. Results indicated that five genotypes (AA, BB, CC, CD and DD) were found in the first intron with one deletion site and two mutant sites (T1003del, G1022A and T1063G), two genotypes (EE and EF) were found in the third exon with one mutant site (T132C). The individuals with genotypes AA had a lager value than the individuals with the genotype CD and DD in total length, body length, body height, body width, head length and body weight (P<0.05). The female individuals with genotypes AA also had a lager value than the female individuals with genotypes DD in total length, body length, body height, body width, caudal peduncle height, caudal peduncle width and body weight (P<0.05). This implies that genotype AA has a positive influence on the growth traits of female yellow catfish, and genotype DD is opposite. Genotypes AA and DD can be used in marker assisted selection of female yellow catfish breeding in the future.

Key words: yellow catfish, MSTN, mutant site, polymorphism, growth traits