新一代测序技术应用于microRNA检测

doi:10.3724/SP.J.1005.2012.00784

遗传 ›› 2012, Vol. 34 ›› Issue (6): 784-792.doi: 10.3724/SP.J.1005.2012.00784

• 技术与方法 • 上一篇

新一代测序技术应用于microRNA检测

汤海明¹, 陈红¹, 张静¹, 任景怡¹, 许宁²

1. 北京大学人民医院心内科, 北京 100044 2. 瑞典卡罗林斯卡医学院医学系, 斯德哥尔摩 17176

收稿日期:2011-10-10 修回日期:2012-12-03 出版日期:2012-06-20 发布日期:2012-06-25
通讯作者: 陈红 E-mail:chenhong0418@yahoo.com.cn
基金资助:
中华医学会临床医学科研专项基金(编号：09010060161)资助

Application of next generation sequencing in microRNA detection

TANG Hai-Ming¹, CHEN Hong¹, ZHANG Jing¹, REN Jing-Yi¹, XU Ning²

1. Department of Cardiology, Peking University People’s Hospital, Beijing 100044, China 2. Department of Medicine, Karolinska Institute, Stockholm 17176, Sweden

Received:2011-10-10 Revised:2012-12-03 Online:2012-06-20 Published:2012-06-25

摘要/Abstract

摘要： MicroRNAs(miRNAs)是一类在进化上高度保守的非编码小分子单链RNA(~22nt), 在基因转录后调控中发挥至关重要的作用。越来越多的证据表明, miRNAs参与很多重要的生理和病理过程, 例如发育、器官形成、调亡、细胞增殖、肿瘤发生等。近年来飞速发展的新一代测序技术在miRNA检测方面具有重要的应用。文章简要介绍了新一代测序技术3大平台的基本步骤和原理, 测序数据的生物信息学分析方法以及新一代测序技术在miRNA方向的主要应用。相比于传统的miRNA检测方法, 新一代测序技术具有通量高、对遗传物质检测完全且准确度高, 可重复性好等优点, 在探索新miRNA、miRNA互补链、miRNA编辑、miRNA异构体检测以及miRNA靶基因检测等方面具有巨大优势。随着新一代测序技术的不断发展, 测序成本不断降低, 在未来几年, 新一代测序技术的使用率或将大大增加。新一代测序技术的不断应用将进一步促进人类对于miRNA在各种生理病理过程中的功能和调控的认识。

关键词: miRNA靶基因检测, microRNAs(miRNAs)检测, 新一代测序技术, 新miRNA检测

Abstract: MicroRNAs (miRNAs) are a class of ~22nt long non-coding RNAs. They are evolutionarily conserved and play essential roles in the regulation of post-transcriptional gene expression. The rapidly developing next generation sequencing (NGS) has important applications in miRNA detection. This review is focused on the mechanism of three NGS platforms and their applications in miRNA detection. In contrast to traditional methods, NGS has major advantages: high throughput, precise, accurate, and repeatable. Its application includes new miRNAs exploration, detection of miRNA*, miRNA editing, and isomiR and target mRNA detection. As NGS develops, the cost of sequencing is declining which makes it possible for NGS to be widely used in the coming years. Next generation sequencing will greatly promote researches of miRNAs.

Key words: new miRNAs explo-ration, target mRNA detection, high throughput detection of microRNAs (miRNAs), next generation sequencing

汤海明，陈红，张静，任景怡，许宁. 新一代测序技术应用于microRNA检测[J]. 遗传, 2012, 34(6): 784-792.

TANG Hai-Ming, CHEN Hong, ZHANG Jing, REN Jing-Yi, XU Ning. Application of next generation sequencing in microRNA detection[J]. HEREDITAS, 2012, 34(6): 784-792.

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新一代测序技术应用于microRNA检测

Application of next generation sequencing in microRNA detection

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