绵羊X染色体59571364与59912586位点在脂尾、瘦尾绵羊群体中的多态性检测及分析

doi:10.3724/SP.J.1005.2013.01384

遗传 ›› 2013, Vol. 35 ›› Issue (12): 1384-1390.doi: 10.3724/SP.J.1005.2013.01384

绵羊X染色体59571364与59912586位点在脂尾、瘦尾绵羊群体中的多态性检测及分析

张伟^1,2,3, 沈敏¹, 李欢¹, 高磊¹, 梁耀伟¹, 杨井泉¹, 刘守仁¹, 王新华¹, 甘尚权¹

1. 新疆农垦科学院畜牧兽医研究所, 石河子 832000;
2. 新疆农业职业技术学院动物科技学院, 昌吉 831100;
3. 石河子大学动物科技学院, 石河子 832003

收稿日期:2013-07-22 修回日期:2013-11-04 出版日期:2013-12-20 发布日期:2013-11-21
通讯作者: 王新华, 研究员, 研究方向：分子遗传学。E-mail: wangxinhua5751@163.com 甘尚权, 副研究员, 研究方向：绵羊分子育种设计。E-mail: shangquangan@163.com E-mail:wangxinhua5751@163.com
作者简介:张伟, 博士, 讲师, 研究方向：动物育种与繁殖。Tel: 13779256843; E-mail: zhangweigsau@163.com
基金资助:
国家重点基础研究发展计划(“973”计划)前期专项项目(编号：2011CB111501)和国家自然科学基金委-新疆联合重点项目(编号：U1130302)资助

Detection and analysis of polymorphisms of 59571364 and 59912586 loci on X chromosome in fat-tail and thin-tail sheep flocks

ZHANG Wei^1,2,3, SHEN Min¹, LI Huan¹, GAO Lei¹, LIANG Yao-Wei¹, YANG Jing-Quan¹, LIU Shou-Ren¹, WANG Xin-Hua¹, GAN Shang-Quan¹

1. Institute of Animal Husbandry and Veterinary, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, China;
2. College of Animal Science and Technology, Xinjiang Vocational College of Agriculture, Changji 831100, China;
3. College of Animal Science and Technology, Shihezi University, Shihezi 832003, China

Received:2013-07-22 Revised:2013-11-04 Online:2013-12-20 Published:2013-11-21

摘要/Abstract

摘要：

为了探讨绵羊X染色体上两处SNP(59571364和59912586)与绵羊尾脂沉积性状的关系, 继而为利用分子标记辅助选择育种技术培育低脂绵羊品种提供依据, 文章以尾型极端差异的阿勒泰羊、湖羊、中国美利奴细毛羊以及萨福克羊为研究对象, 利用PCR-RFLP检测两位点在群体中的多态性, 并分析了两个SNP位点在阿勒泰羊群体中的单倍型。结果表明：59571364位点的TT基因型和59912586位点的GG基因型在瘦尾中国美利奴与萨福克羊群体中属于优势基因型, 而两种基因型在脂尾(臀)阿勒泰羊与湖羊群体中比率均不足2%; 两位点在阿勒泰羊群体中的单倍型分析结果显示, CA单倍型为主单倍型, 比率高达55%, CA与TA两单倍型约占88.33%。以上结果提示, 绵羊X染色体59571364与59912586位点在脂尾(臀)与瘦尾绵羊群体中分布存在较大差异, 可作为理想的分子标记应用于高、低脂绵羊品种选育。

关键词: 脂尾性状, 阿勒泰羊, SNP位点, 多态性, 基因型

Abstract:

In order to analyze the correlation of tail fat deposition and two SNP loci on Ovis arise chromosome X and provide a theoretical basis for using molecular assisted selection technology in further low-fat sheep breeding, the breeds with extreme differences in tail types (Altay, Small Tail Han Sheep, Hu, Chinese Merino and Suffolk) were used to detect the polymorphisms of two SNP loci on X chromosome and the haplotypes with PCR-RFLP method. The results showed that the TT genotype at 59571364 locus and GG genotype at 59912586 locus were preponderant genotypes in thin-tailed Chinese Merino and Suffolk sheep flocks, while the percentage of the two genotypes in fat-tailed (fat-rmup) Altay and Hu flocks is less than 2%. Haplotype analysis showed that CA haplotype is the main haplotype, the percentage of CA is up to 55%, and the percentage of CA and TA haplotypes together was 88.33% in Altay sheep flock. These results suggest that there are great differences in the SNP distribution of the 59571364 and 59912586 loci among different tail-typed sheep flocks, which can be used as molecular markers in high or low fat sheep breeding.

Key words: fat-tail trait, Altay sheep, SNP locus, polymorphism, genotype

张伟沈敏李欢高磊梁耀伟杨井泉刘守仁王新华甘尚权. 绵羊X染色体59571364与59912586位点在脂尾、瘦尾绵羊群体中的多态性检测及分析[J]. 遗传, 2013, 35(12): 1384-1390.

ZHANG Wei, SHEN Min, LI Huan, GAO Lei, LIANG Yao-Wei, YANG Jing-Quan, LIU Shou-Ren, WANG Xin-Hua, GAN Shang-Quan. Detection and analysis of polymorphisms of 59571364 and 59912586 loci on X chromosome in fat-tail and thin-tail sheep flocks[J]. HEREDITAS, 2013, 35(12): 1384-1390.

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绵羊X染色体59571364与59912586位点在脂尾、瘦尾绵羊群体中的多态性检测及分析

Detection and analysis of polymorphisms of 59571364 and 59912586 loci on X chromosome in fat-tail and thin-tail sheep flocks

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