遗传 ›› 1996, Vol. 18 ›› Issue (1): 29-32.

• 论文 • 上一篇    下一篇

人胚细胞提取物抗突变作用的研究

薛开先1; 吴建中1; 马国建1; 陈森清1; 王瑚2; 向立平2; 程宁2 XUE Kai-Xian1;WU Jian-Zhong1;MA Guo-Jian1;WANG Hu2;XIANG Li-Ping2;CHENG Ning2   

  1. 1.江苏省肿瘤防治研究所, 南京 210009 2.河海大学生物化学研究中心,南京 210024 1.Cancer Institute of Jiangsu Province,Nanjing 210009 2.Biochemical Research Center,Hehai University,Nanjing 210024
  • 收稿日期:1900-01-01 出版日期:1996-02-10 发布日期:1996-02-10

Studies on Antimutagenic Effects of Human Fetal Cell Extracts

  • Received:1900-01-01 Online:1996-02-10 Published:1996-02-10

摘要: 本文应用体外培养人淋巴细胞和小鼠骨髓微核与染色体畸变检测,研究了人胚细胞水提物(胚提物)的遗传毒性和抗突变效应。结果表明,(1)胚提物(3和30μg/ml,下同)可显著抑制培养人淋巴细胞的自发和γ-射线诱发的微核形成;(2)胚提物对培养淋巴细胞的自发染色体畸变无明显影响,但可显著抑制丝裂酶素C(MMC)诱发的染色体畸变; (3)胚提物显著抑制环磷酰胺诱发的小鼠骨髓多染性红细胞微核(PCE-MN)的增加; (4)胚提物的上述抗突变效应呈剂量依赖性增加。因此,作者认为,人胚细胞水提物是一种抗突变剂, 可望用于肿瘤的化学预防,并可用作为肿瘤放疗和化疗的辅助药物,以减少毒付反应和二次肿瘤的发生率。
Abstract:Genetially toxic and antimutagenic effects of the aqueous extracts of human fetal cells(HFCAE) against mutagen-induced chromosomal aberrations and micronucleus formation in human lymphocytes in vitro and bone marrow of mice were studied.HFCAE (3 and 30 μg/ml)significantly inhibited spontaneous and γ-rays-induced micronucleus formation and MMC-induced chromosomal aberrations in human lymphocytes in vitro.HFCAE (3 and 30μg/ml) strongly suppressed also micronucleus formation induced by CP in PCEs of mice.These antimutagenic effects of HFCAE were dose-dependent.Our results suggest that HFCE might be an antimutagen and have potential value in its clinical application.

关键词: 微核
Key words,
Aqueous extracts of human fetal cells, 染色体畸变, 人胚细胞水提物, 抗突变剂, PEG法, 下胚轴, 原生质体培养, 转基因植株, 诸葛菜

Key words: Orychophragmus violaceus, Hypocoyl Protoplast culture PEG Transgenic plant