遗传 ›› 2001, Vol. 23 ›› Issue (3): 226-228.

• 论文 • 上一篇    下一篇

JL-02多位点探针DNA指纹在近交系小鼠遗传监测中的应用

董罡1;陈振文2;陈松2;李继周3;郭红玲3;季安全3 DONG Gang1;CHEN Zhen-wen2;CHEN Song2;LI Ji-zhou3;GUO Hong -ling3;JI An-quan3   

  1. 1.中国人民解放军总医院医学实验动物中心,北京 100853; 2.军事医学科学院实验动物中心,北京 100038; 3.公安部物证鉴定中心,北京 100071 1.Laboratory Animal Center of the General Hospital of P.L.A. BeiJing 100853; 2.Laboratory Animal Center of Academy of Military Medical Sciences BeiJing 100038; 3.The Public Safety Bureau of China Bei Jing 100071,China
  • 收稿日期:1900-01-01 出版日期:2001-06-10 发布日期:2001-06-10

Use of Probe JL-02 in Fingerprinting in Inbred Mice Ge netic Monitor

  • Received:1900-01-01 Online:2001-06-10 Published:2001-06-10

摘要: 以公安部二所研制的JL-02多位点探针进行了BALB/C、C57、DBA/2、615和ICR5个品系近交系小鼠DNA指纹分析。结果表明,不同近交系小鼠的DNA指纹图差异很大,而同一品系DNA指纹图基本一致,从而证明这一新探针适用于利用DNA指纹技术对近交系小鼠进行遗传监测并具有高度多态性。
Abstract:To explore the possibility of using the probe JL-02 by the Public Safety Bureau of China in monitoring genetic quality of inbred mic e. Fingerprinting of 5 strains of the inbred mice (BALB/c、C57、DBA/2、615、ICR) was conducted using a nonisotopically HRP labeled JL-02 and southern blot hybri dization. The patterns were completely different among the 5 strains of the mice and those of the samples from the same strains were completely identical and th e probe has the high polymorphism.

关键词: DNA fingerprinting, 近交系, 小鼠 Keywords, DNA指纹, 遗传监测