一种限制性cDNA文库的构建

遗传 ›› 2002, Vol. 24 ›› Issue (2): 174-176.

一种限制性cDNA文库的构建

祝骥1;2;马文丽2;李凌2;姚汝华1;郑文岭3 ZHU Ji1;2;MA Wen-li2;LI Ling2;YAO Ru-hua1;ZHENG Wen-ling3

1.华南理工大学生物工程系,广州 510641; 2.第一军医大学分子生物学研究所,广州 510515; 3.广州军区广州总医院分子肿瘤研究所,广州 510010 1.Department of Bioengincering,South China University of Techndogy,Guangzhou 510640,China; 2.Institute of Molacular Biology,First Military Medical University,Guangzhou 510515,China; 3.Liu Hua Qiao Hospital,Medical Center,Guangzhou 510010,China

收稿日期:1900-01-01 出版日期:2002-04-10 发布日期:2002-04-10

A Method for Construction of Restriction cDNA Library

Received:1900-01-01 Online:2002-04-10 Published:2002-04-10

摘要/Abstract

摘要： 利用本室创建的限制性显示技术RD-PCR,建立的cDNA文库,我们称之为限制性cDNA文库。该方法构建的文库因经过了限制性分组扩增，每组均含有特定的cDNA，因而大大加快了随后克隆的分离和鉴定的速度。
Abstract:A kind of cDNA library constructed according to Restriction Display PCR(RD-PCR) technology setup in our lab,which was called restriction cDNA library,was introduced in this paper.In the construction of cDNA library,cDNA was digested with restriction endonuclease,linked with special adaptor,amplified with PCR in groups.Each group of the restriction cDNA library contained special cDNAs.The method greatly reduced the repetitive frequency and accelerated the speed of identification.

关键词: RD-PCR
Key words, Restriction cDNA library, K562 cell, K562细胞, 限制性cDNA文库

祝骥，马文丽，李凌，姚汝华，郑文岭. 一种限制性cDNA文库的构建[J]. 遗传, 2002, 24(2): 174-176.

ZHU Ji, MA Wen-li, LI Ling, YAO Ru-hua, ZHENG Wen-ling. A Method for Construction of Restriction cDNA Library[J]. HEREDITAS, 2002, 24(2): 174-176.