遗传 ›› 2002, Vol. 24 ›› Issue (3): 263-266.
王秀利1;3;李宁2;赵志辉2;冯继东2;赵兴波1;李长绿1;吴常信1 WANG Xiu-li1;3;LI Ning2;ZHAO Zhi-hui2;FENG Ji-dong2;ZHAO Xing-bo1;LI Chang-lv1;WU Chang-xin1
摘要: 以香猪背最长肌为实验材料,以Lambda ZAP II为载体,构建了肌肉组织的cDNA文库。结果表明,cDNA文库的滴度在3.4×107 pfu/ml以上,重组率在94%以上,插入片段大小平均在1.5kb以上。同时指出,如果从3′端测序,多于30 个碱基T的插入片段是造成ESTs测序成功率低的主要因素。
Abstract:Using Longissimus Dorsi muscle as material and Lambda ZAP II as Vector,Xiang Pig Longissimus Dorsi muscle cDNA library has been constructed in our study.The results showed that the tritation of the library was 3.4×107 pfu/ml,the recombinant percentage was 94%,and the fragment length of inserted average cDNA were 1.5kb.The study pointed out that the more than 30 T insertion is the major factor for low percetage if sequencing the 3′-end.