摘要： 从14.5~16.5d的大鼠胚胎分离神经干细胞，培养于添加相应成分以及表皮生长因子（EGF）和碱性成纤维细胞生长因子（bFGF）的DMEM/F12培养液中。通过神经干细胞球直接计数和3H胸苷掺入两种方法研究脂类对大鼠胎儿神经干细胞(rFNSCs)生长和增殖的影响，结果显示，脂类不但可以增加神经干细胞的数量，而且可以增加3H胸苷掺入率，表明脂类对大鼠胎儿神经干细胞的生长和增殖有一定的促进作用，可以作为神经干细胞培养的添加成分。

Studies of Effects of Lipid on Rat Fetal Neural Stem Cells
LI Xue-ling1,HU Ting-mao1,Zhalagahu1,YU Hai-quan1,John R.Morrison2
1.Faculty of Life Science,Neimenggu University,Huhhot 010021,China；
2.Monash Institute of Reproduction and Development,Clayton VIC 3168,Australia
Abstract:Rat fetal neural stem cells (rFNSCs) was separated from embryo about 14.5~16.5 days,and cultured in DMEM/F12 media with additives and epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF).Effects of lipid on growth and proliferation of rFNSCs was examined by counting the number of neurospheres and incorporation of 3H.The data show that chemical defined lipid improved rFNSCs' growth and cell division.Lipid will be another neural stem cell's culture media's additive.
Key words：rat fetal neural stem cells (rFNSCs); lipid

关键词: 脂类, 大鼠胎儿神经干细胞(rFNSCs)