遗传 ›› 2003, Vol. 25 ›› Issue (6): 633-636.

• 论文 • 上一篇    下一篇

人类生殖相关新基因的定位和组织表达

罗阳1;2;于秉治2 LUO Yang1;2;YU Bing-Zhi2   

  1. 1.中国医科大学卫生部细胞生物学重点实验室,医学基因组学研究室,沈阳 110001; 2.中国医科大学生物化学教研室,沈阳 110001 1.The Research Center for Medical Genomics and MOH Key Laboratory of Cell Biology,China Medical University,Shenyang 110001,China; 2.Department of Biochemistry,China Medical University,Shenyang 110001,China
  • 收稿日期:1900-01-01 出版日期:2003-12-10 发布日期:2003-12-10

Gene Localization and Tissue Expression of a Novel Human Reproduction-related Gene

  • Received:1900-01-01 Online:2003-12-10 Published:2003-12-10

摘要: 基因定位对研究基因之间以及基因与疾病之间的相互关系具有重要意义。应用辐射杂种细胞系技术(RH)对我们克隆的人类新基因HBRP(Human BSP-Related Protein)进行了染色体定位,结果将该基因定位于19q13.2~13.3,同时应用生物信息学方法在人类基因组重叠片段数据库进行该基因的定位,结果相吻合。研究证明,RH技术具有快速、精确、简便等优点,是基因定位研究中一强有力的技术。同时通过RT-PCR方法研究了HBRP基因在人体各组织中的表达分布,结果显示该基因在睾丸、肠、肾、肝、脾、胃、胰腺组织有较高的表达,而在检测的脑、肺、骨骼肌、心肌组织中表达较弱。
Abstract:Gene localization is significant in elucidating the interaction between genes,gene and diseases.Using radiation hybrid (RH) technique,we cloned and localized a novel gene,designated human BSP-related protein (HBRP) on 19q13.2~13.3,in line with its localization in data bank of overlapping fragment of human genome through bioinformatics method.It is suggested RH is rapid,precise,simple and powerful in gene localization.In addition,we detected the expression and distribution of HBRP in human tissues by RT-PCR.The results showed HBRP was highly expressed in intestine,kidney,liver,spleen,stomach and pancreas,whereas lowly in brain,lung,muscle and heart.

关键词: HBRP, 生物信息学
Key words,
RT-PCR, 辐射杂种细胞系, 染色体定位