摘要：

常规方法分离鉴定47株大肠埃希菌，以标准纸片扩散法(KB法)对其进行常用氨基糖苷类药物敏感性测定，耐药株经PCR检测aac(3)Ⅱ基因保守区，扩增产物进行DNA测序分析。初步探讨大肠埃希菌氨基糖苷类抗生素耐药株与aac(3)Ⅱ基因保守区之间的关系，结果显示本地区大肠埃希菌氨基糖苷类抗生素耐药株的aac(3)Ⅱ基因保守区具65位G、84位T和65位A、84位C两种基因型，且高度耐药菌株皆为65位G、84位T基因型。Abstract:According to standard KB method,bacteriostatic tests were performed to screen out aminoglycoside resistance bacteria from 47 strains of isolated E.coli.To analyze correlations between the degree of E.coli aminoglycoside resistance and aac(3)Ⅱgene conserved region,PCR amplified aac(3)Ⅱgene conserved regions and were analyzed by DNA sequencing.The results showed that there were two species of aac(3)Ⅱgene type including 65G and 84T or 65A and 84C in the samples.Strains with high activity of modifying enzyme to gentamicin all were 65G and 84T aac(3)Ⅱgene type.