遗传 ›› 2005, Vol. 27 ›› Issue (1): 91-94.

• 论文 • 上一篇    下一篇

中国蒙古马与国外纯血马mtDNA D-Loop高变区序列比较

芒 来;李金莲;石有斐MANG Lai;LI Jin-Lian;SHI You-Fei   

  1. 内蒙古农业大学动物科学与医学学院,呼和浩特 010018 College of Animal Science and Veterinary,Inner Mongolia Agricultural University,Huhhot 010018,China
  • 收稿日期:1900-01-01 出版日期:2005-01-28 发布日期:2005-01-28

Sequence Comparing of mtDNA D-Loop Varied Region in Chinese Mongolian Horse and External Thoroughbred Horse

  • Received:1900-01-01 Online:2005-01-28 Published:2005-01-28

摘要: 比较分析了4匹中国蒙古马和4匹国外纯血马的线立体DNA(mtDNA)D-Loop高变区400bp核苷酸序列的变异情况。结果发现,4匹中国蒙古马mtDNA D-Loop高变区的平均核苷酸变异率为3.69%,而纯血马的为4.00%,其核苷酸变异类型均包括转换、颠换和缺失3种形式,其中以转换最为常见。核苷酸变异基因座多,并且存在长度变异,不同变异在个体之间差异也很大,因此说明中国蒙古马和国外纯血马的mtDNA D-Loop高变区都具有丰富的多态性。Abstract:Mitochondrial DNA D-Loop varied region 400bp sequence variations in 4 Chinese Mongolian horses and 4 External Thoroughbred horses were analyzed in this experiment. The results showed that the average nucleotide mutational rate of mtDNA D-Loop varied region in 4 Chinese Mongolian horses was 3.69%,while External Thoroughbred horses were 4.00%. Three types of mutations including transition,transversion and deletion were all found in the investigated mtDNA D-Loop regions,of which transition was the most frequent. Nucleotide mutational loci were abundant,length mutations were found and great differences were all observed among the 8 horses. It showed there existed much polymorphism in the mitochondrial DNA D-Loop varied region of Chinese Mongolian horses and External Thoroughbred horses.

关键词: 国外纯血马, mtDNA D-Loop, 高变区, 中国蒙古马, 关键词