遗传 ›› 2005, Vol. 27 ›› Issue (3): 377-381.

• 研究报告 • 上一篇    下一篇

RAPD标记鉴定水稻3种不同细胞质雄性不育类型的杂交种及其亲本

徐 鑫1;2;刘学群1;2;瞿 波 3;朱英国2;王春台1   

  1. 1.中南民族大学生物技术国家民委重点实验室,武汉 430074; 2.武汉大学植物发育教育部重点实验室,武汉 430072; 3.华中农业大学植物科学技术学院,武汉 430070
  • 收稿日期:2004-06-14 修回日期:2004-12-15 出版日期:2005-06-10 发布日期:2005-06-10
  • 通讯作者: 王春台

RAPD Identification of Three Types of Three-lines Hybrid Rice with Different Male Sterile Cytoplasms and Their Parent Lines

XU Xin 1,2;LIU Xue-Qun1,2;QU Bo 3;ZHU Ying-Guo2;WANG Chun-Tai1   

  1. 1. Biotechnological Laboratory of National Commission for Nationalities, College of Chemistry and Life Science, South–central University for Nationalities, Wuhan 430074,China; 2. Key Laboratory of MOE for Plant Developmental Biology, College of Life Science, Wuhan University, Wuhan 430072 China; 3.College of Plant Science and Technology, Huazhong Agricultural University,Wuhan 430074 China
  • Received:2004-06-14 Revised:2004-12-15 Online:2005-06-10 Published:2005-06-10
  • Contact: WANG Chun-Tai

摘要: 利用RAPD引物对3种不同细胞质雄性不育类型的杂交水稻组合及其亲本共21个材料进行了DNA多态性分析。从264个随机引物中筛选出具有非常明显多态性的引物25个, 对25个引物在3种不同细胞质不育类型的杂交组合及其亲本间的DNA扩增多态性差异进行比较,最终选出具有不同类型间特异性扩增带的引物7个,利用这些特异性扩增带能有效地区分和鉴定目前在生产上大面积种植和推广,或者是具有应用潜力的3种不同细胞质雄性不育类型—野败型(WA)、红莲型(HL)和包台型(BT))—的6种杂交水稻组合及其亲本。

关键词: 多态性, 细胞质雄性不育, 杂交水稻, RAPD

Abstract: The RAPD analysis was conducted on genome DNA from 21 sets of rice, including 6 three–line hybrid rice combinations, separately derived from three different kinds of cytoplasmic male sterile (CMS) lines and their related parents. Out of 264 random primers screened first, 25 primers displayed well in polymorphisms. It was shown that only 7 bands amplified respectively from 7 primers were enough to discriminate the different types of CMS, the hybrid combinations and their parents.

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