遗传 ›› 2005, Vol. 27 ›› Issue (6): 903-907.

• 研究报告 • 上一篇    下一篇

微卫星DNA在吉戎兔亲子鉴定中的应用研究

韩春梅1;张嘉保2 ;高庆华1;陈庆波3   

  1. 塔里木大学动科院,新疆843300
  • 收稿日期:2004-10-25 修回日期:2005-02-25 出版日期:2005-12-10 发布日期:2005-12-10
  • 通讯作者: 张嘉保

Study on Parentage Testing in JIRONG Rabbit by Microsatellite Markers

HAN Chun-Mei1, ZHANG Jia-Bao2, GAO Qing-Hua1, CHEN Qing-Bo3   

  1. College of Animal Science and Technology, Talim University, Alar, Xingjiang Autonomous Region, Xinjiang 843300,China
  • Received:2004-10-25 Revised:2005-02-25 Online:2005-12-10 Published:2005-12-10
  • Contact: zhjb515@263.com

摘要: 选用Sat2、Sat3、Sat4、Sat5、Sat7、Sat8、Sat12、Sat13、Sat16、Sol08、Sol28、Sol30、Sol03 等13个微卫星位点PCR扩增30只吉戎兔的基因组DNA,然后在8%聚丙烯酰胺凝胶上电泳分型,检测结果为平均等位基因数为3.46个,平均杂合度(H)为0.578,平均多态信息含量(PIC)为0.531,双亲资料未知时13个位点的累计非父排除率(PE1)为0.935226,置信度低于80%,一亲本资料已知时13个位点的累计非父排除率(PE2)为0.999329,置信度为95%。由于所鉴定兔群的母本资料已知,因此基因型的分型结果能有效确认18只仔兔子的真父。

关键词: 吉戎兔, 微卫星DNA, 亲子鉴定

Abstract: Thirteen microsatellite markers, including Sat2,Sat3,Sat4,Sat5,Sat7,Sat8,Sat12, Sat13, Sat16,Sol08,Sol28,Sol30 and Sol03, were studied for their parentage-testing application in a group of 30 JIRONG Rabbits in the present report. The 13 microsatellite loci were successfully amplified with specific primers designed according to known sequences. The PCR products amplified from the microsatellite loci were analyzed by 8% denaturing polyacrylamide gel electrophoresis. The results demonstrated that the average alleles and the mean heterozygosity (Hs) and the polymorphism information content (PIC) and the combined exclusion probability (PE2) of the 13 microsatellite loci were 3.46, 0.578, 0.531, and 0.999329, respectively. The exclusion probability (PE1) of the 13 loci was 0.935226, and the confidence of the parentage testing was less than 80% when data of both parents were unknown, while the exclusion probability (PE1) and the confidence were 0.999329 and 95% respectively with known data of a single parent. Since the data of the rabbit maternal lines studied were known, the paternal lines of the group were successfully identified using the 13 microsatellite loci with high confidence.

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