微卫星DNA监控大鼠近交系的培育

遗传 ›› 2006, Vol. 28 ›› Issue (7): 821-824.

微卫星DNA监控大鼠近交系的培育

李瑞生1, 董罡1, 吴晓燕1 , 王鹏1 , 王晓辉2, 陈振文2

1．军事医学科学院实验动物中心, 北京 100071；2．首都医科大学实验动物科学部, 北京 100054

收稿日期:2005-06-26 修回日期:2005-11-02 出版日期:2006-07-10 发布日期:2006-07-10
通讯作者:
陈振文

Inbred Cultivation of Microsatellite DNA Monitoring Outbred Rats

LI Rui-Sheng1, DONG Gang1, WU Xiao-Yan1, WANG Peng1, WANG Xiao-Hui2,
CHEN Zhen-Wen2

1. Laboratory Animal Center, Academy of Military Medical Sciences，Beijing 100071, China；
2. Department of Laboratory Animal Science, Capital University of Medical Sciences,Beijing 100054,China

Received:2005-06-26 Revised:2005-11-02 Online:2006-07-10 Published:2006-07-10
Contact:
CHEN Zhen-Wen

摘要/Abstract

摘要： 采用微卫星DNA技术来监控大鼠仔代基因状况，选择性地进行交配繁殖，使基因快速纯合，缩短培育新的近交系动物周期。利用PCR扩增30个微卫星DNA位点对封闭群SD和Wistar大鼠交配繁殖的仔代鼠进行微卫星DNA多态性分析，仔代中与母代SD大鼠相似系数高的与中的进行定向交配繁殖。F2代大鼠均为杂合多态的位点，没有纯合位点；到F9代时基因纯合位点达27个，纯合基因位点率为90%。每代相似系数具有不断上升的趋势，上升率为6-20%。采用皮肤移植方法验证了F9代大鼠间无排斥。建立了一种新的快速培育近交系动物的方法。

关键词: 大鼠, 微卫星DNA, 封闭群, 近交系培育

Abstract: It was monitored heredity gene of baby rats with microsatellites DNA. And selected to breed rats, make gene quickly identical, and it would shorten cultivated periods of a new inbred rats. It were analyzed DNA polymorphism in baby rats between outbred Wistar and SD rats with 30 microsatellites DNA loci of PCR amplification. And was breeding baby inbred rats using high and middle of Coefficient of similarity with female SD rats of F0. It was indicated that there were hybrid and polymorphism loci, there weren’t homo-gene loci of F2. It were monomorphism homo-gene for 27, rate of homo-gene loci for 90%. It was unceasing rised for coefficient of similarity of every generation, rate of rising for 6-20%. And it was proved that it was not excluded between individual of F9 generation with skin transplantation method. It established a new method of quickly cultivation inbred rats.

中图分类号:

Q953

李瑞生，董罡，吴晓燕，王鹏，王晓辉，陈振文. 微卫星DNA监控大鼠近交系的培育[J]. 遗传, 2006, 28(7): 821-824.

LI Rui-Sheng, DONG Gang, WU Xiao-Yan, WANG Peng, WANG Xiao-Hui, CHEN Zhen-Wen . Inbred Cultivation of Microsatellite DNA Monitoring Outbred Rats[J]. HEREDITAS, 2006, 28(7): 821-824.

[1]	刘梅珍, 王立人, 李咏梅, 马雪云, 韩红辉, 李大力. 利用CRISPR/Cas9技术构建基因编辑大鼠模型[J]. 遗传, 2023, 45(1): 78-87.
[2]	翟伟, 胡宏秀, 乐亮, 庄峰峰, 王克柱, 赵英, 王凯, 刘新民, 孙迪安, 王晓英, 匡世焕, 胡克平. Rett综合征相关基因MeCP2敲除大鼠模型的构建及分析[J]. 遗传, 2016, 38(11): 1004-1011.
[3]	汤晓丽，邓立彬，李桂林，刘双梅，林加日，谢金燕，刘俊，孔繁君，梁尚栋. 2型糖尿病早期大鼠外周神经节基因表达谱分析[J]. 遗传, 2012, 34(2): 198-207.
[4]	王泽，文园园，程振朝，郭学强，张新胜，徐存拴. 新基因BM390716、BI274487和AA963863与大鼠再生肝8种细胞的细胞外基质代谢[J]. 遗传, 2011, 33(4): 378-388.
[5]	丁贤明，钱宝珍，Matsuda Junichiro，Koura Minako，萨晓婴，施张奎. 长爪沙鼠的遗传多样性分析[J]. 遗传, 2008, 30(7): 877-884.
[6]	韩春梅，张嘉保，高庆华，陈庆波. 微卫星DNA在吉戎兔亲子鉴定中的应用研究[J]. 遗传, 2005, 27(6): 903-907.
[7]	方晓敏，许尚忠，张英汉，徐宁迎. 西门塔尔牛产奶性状的微卫星标记分析[J]. 遗传, 2005, 27(4): 571-574.
[8]	张会勇，李玉昌，林俊堂，徐存拴. 大鼠热激因子结合蛋白1全长cDNA克隆与分析Cloning[J]. 遗传, 2004, 26(5): 647-652.
[9]	张英杰，赵有璋，刘月琴，李玉，孙少华，孙洪新. 3个山羊群体中4个微卫星DNA多态性及其与杂种优势的关系[J]. 遗传, 2004, 26(5): 631-636.
[10]	高军，任军，陈克飞，黄路生，Bertram Brenig. 猪PAC克隆的微卫星DNA分离研究[J]. 遗传, 2003, 25(6): 660-662.
[11]	李雪玲，扈廷茂，扎拉嘎胡，于海泉,John RMorrison. 脂类对大鼠胎儿神经干细胞生长和增殖的影响 [J]. 遗传, 2002, 24(6): 663-666.
[12]	陈振文，李瑞生，王承利，赵林山，宋德光，王大鹏. 用DNA指纹图技术分析Wistar大鼠的遗传距离[J]. 遗传, 2002, 24(3): 275-278.
[13]	李瑞生，陈振文，宋德光，任文陟，栾蓉晖. PCR扩增近交系大鼠微卫星位点DNA多态性的研究[J]. 遗传, 2001, 23(6): 539-313.
[14]	洪燕敏，杨欣艳，王学瑞，宋学文，吴锐，郑红，张镜宇. 大鼠脑组织中胆囊收缩素基因表达与发育的关系[J]. 遗传, 2001, 23(1): 14-16.
[15]	樊叶杨，庄杰云，吴建利，孙宝龙，郑康乐. 应用微卫星标记鉴别水稻籼粳亚种[J]. 遗传, 2000, 22(6): 392-394.