拟南芥Peptide5和Peptide6的表达谱分析

doi:10.3724/SP.J.1005.2008.01615

遗传 ›› 2008, Vol. 30 ›› Issue (12): 1615-1620.doi: 10.3724/SP.J.1005.2008.01615

拟南芥Peptide5和Peptide6的表达谱分析

蒋细兵^{1, 2}; 余迪求¹

1. 中国科学院西双版纳热带植物园昆明分部, 昆明650223;
2. 中国科学院研究生院, 北京100049

收稿日期:2008-04-08 修回日期:2008-06-06 出版日期:2008-12-10 发布日期:2008-12-10
通讯作者: 余迪求

Expression profile analysis of Peptide5 and Peptide6 in Arabidopsis

JIANG Xi-Bing^{1, 2};YU Di-Qiu¹

1. Kunming Section, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China;
2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China

Received:2008-04-08 Revised:2008-06-06 Online:2008-12-10 Published:2008-12-10
Contact: YU Di-Qiu

摘要/Abstract

摘要：

摘要: 通过对5个拟南芥(Arabidopsis thaliana L.)预测性多肽进行RT-PCR分析, 在mRNA水平证实了Peptide5和Peptide6预测性多肽的真实性。表达谱分析表明: 两基因在不同的发育期和不同的组织普遍表达, 为组成型基因; 对NaCl、聚乙二醇4000(PEG4000)、茉莉酸甲酯(MeJA)、水杨酸(SA)、机械损伤和冷害做出基因转录水平的响应。启动子顺式作用元件分析提示, 拟南芥Peptide5基因可能参与了次生木质部的形成。

关键词: 预测性多肽基因, Peptide5, Peptide6, 表达谱, 顺式作用元件分析

Abstract:

Abstract: Two predicted peptide genes in Arabidopsis thaliana L., Peptide5 and Peptide6, was confirmed by RT-PCR in mRNA level. The expression profile indicated that both genes were generally expressed at different developmental stages and tissues as constitutive gene expression, and they also responded to six treatments including NaCl, PEG, MeJA (methyl jasmonate), SA (salicylic acid), cold and wound in transcription level. Analysis of the promoter sequence suggests that Pep-tide5 in Arabidopsis may contribute to the secondary xylem formation.

蒋细兵，余迪求 . 拟南芥Peptide5和Peptide6的表达谱分析[J]. 遗传, 2008, 30(12): 1615-1620.

JIANG Xi-Bing, YU Di-Qiu. Expression profile analysis of Peptide5 and Peptide6 in Arabidopsis[J]. HEREDITAS, 2008, 30(12): 1615-1620.

拟南芥Peptide5和Peptide6的表达谱分析

Expression profile analysis of Peptide5 and Peptide6 in Arabidopsis

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