遗传 ›› 2008, Vol. 30 ›› Issue (6): 776-780.doi: 10.3724/SP.J.1005.2008.00776

• 研究报告 • 上一篇    下一篇

元江普通野生稻叶片cDNA文库的构建及部分基因片段分析

史冬燕1, 2, 杨明挚1, 黄兴奇3, 陈善娜1, 程在全3   

  1. 1. 云南大学生命科学学院, 昆明 650091;
    2. 山东菏泽学院生物系, 菏泽 274015;
    3. 云南省农科院生物技术与种质资源研究所, 昆明 650203

  • 收稿日期:2007-11-22 修回日期:2007-12-20 出版日期:2008-06-10 发布日期:2008-06-10
  • 通讯作者: 程在全

Construction and analysis of cDNA library of Yunnan Yuanjiang O. rifupogon leaf

SHI Dong-Yan1, 2, YANG Ming-Zhi1, HUANG Xing-Qi3, CHEN Shan-Na1,
CHENG Zai-Quan3   

  1. 1. School of Life Science, Yunnan University, Kunming 650091, China;
    2. Biology Department of Heze College, Heze, Shansong Province 274015, China;
    3. Biotechnology & Genetic Germplasm Institute, Yunnan Academy of Agricultural Sciences, Kunming 650203, China
  • Received:2007-11-22 Revised:2007-12-20 Online:2008-06-10 Published:2008-06-10
  • Contact: CHENG Zai-Quan

摘要:

首次利用SMARTTM技术构建了中国普通野生稻中最原始类型——元江普通野生稻生长旺盛时期叶片的cDNA文库。该cDNA文库未扩增和扩增后的滴度分别为1.1×106 pfu/mL和3.98×107 pfu/mL, 重组率为91%, 插入片段大小为500~2 000 bp。测定的部分cDNA序列进行BLAST比较, 发现这些cDNA片段与日本晴栽培稻同源性很高, 达到98%以上。本研究为进一步分析这些cDNA片段的结构、功能和探讨元江普通野生稻在栽培稻演化中的地位奠定了基础。

关键词: cDNA文库, 元江普通野生稻, 滴度

Abstract:

The cDNA library of Yuanjiang Oryza rifupongon leaf was constructed by using SMARTTM technology. The titers of the non-amplified library and the amplified library were 1.1×106 pfu/mL and 3.98×107 pfu/mL, respectively. The recombi-nation rate was more than 91%. The DNA sequence length of the most cDNAs in the library was between 500-2 000 bp. Some cDNAs chosen by random were sequenced. After BLAST analysis of some cDNAs, their possible function were pre-dicted. It is found that these cDNAs show 98% similarity to Oryza sativa japonica in the NCBI database. These provided a base for further study on the structure and function of these cDNAs and evolutionary process of Yuanjiang Oryza rifupongon .