遗传 ›› 2009, Vol. 31 ›› Issue (5): 523-523―530.doi: 10.3724/SP.J.1005.2009.00523

• 研究报告 • 上一篇    下一篇

基于Adh1基因分析高粱属的系统进化关系

廖芳1,2;刘勇1;杨秀丽1;黄国明1;牛春敬1   

  1. 1. 天津出入境检验检疫局, 天津 300456;
    2. 南开大学生命科学学院, 天津 300071

  • 收稿日期:2008-06-12 修回日期:2008-10-06 出版日期:2009-05-10 发布日期:2009-05-10
  • 通讯作者: 黄国明

Molecular phylogenetic relationships among species in the genus Sorghum based on partial Adh1 gene

LIAO Fang1, 2;LIU Yong1;YANG Xiu-Li1;HUANG Guo-Ming1;NIU Chun-Jing1   

  1. 1. Tianjin Entry-Exit Inspection and Quarantine Bureau, Tianjin 300456, China;
    2. College of Life Sciences, Nankai University, Tianjin 300071, China
  • Received:2008-06-12 Revised:2008-10-06 Online:2009-05-10 Published:2009-05-10

摘要: 高梁属中有重要的粮食作物和优良牧草, 也有农业生产上的重要杂草。文章旨在进一步从分子水平阐明高梁属种间的系统进化关系, 为有效利用种质资源进行分子育种改良作物品质提供理论依据, 并明确检疫性杂草的分类地位。根据二色高粱(Sorghum bicolor)的Adh1全基因序列(GenBank登录号: AF050456)设计引物, 扩增并测定黑高粱(S. almum)、假高粱(S. halepense)、丝克高粱(S. silk)和苏丹草(S. sudanense)共计8个植物材料约2 000 bp的Adh1基因部分序列, 结合GenBank中其他24个Sorghum属的同源序列, 以Cleistachne sorghoides的对应序列为外群, 进行了高梁属的亲缘关系分析, 用MP、ML和NJ法分别构建了分子进化树, 得到了基本相同的拓扑结构。结果显示: (1) 高梁属可明显分为三大支, 一支是蒴柄高梁(Chaetosorghum)和异高梁(Heterosorghum)二个亚属, 一支是优高梁亚属(Eusorghum), 这两个分支包含2n=20、40, 染色体较小的种类, 另一分支包括拟高梁 (Parasorghum)和有柄高梁(Stiposorghum)两个亚属, 包含2n=10的种类和它们的多倍体近缘种, 染色体相对较大; (2) S. almum的Adh1基因表现出明显的地理分化; (3) Parasorghum亚属的S. pur-pureosericeum和多色高粱(S. versicolor)、光高粱(S. nitidum)和S. leiocladum聚在一起, 而该亚属中的S. mata-rankense、S. grande、S. timorense却与亚属Stiposorghum的种聚在一起, 表现出更近的亲缘关系; (4) S. mac-rospermumS. laxiflorum之间具有比其他高梁属种更近的亲缘关系。

关键词: 分子系统树, 亲缘关系, Adh1基因, 高梁属

Abstract: The genus Sorghum contains some important grain crops and economically important forage grasses as well as agricultural weeds. The goals of this study were to elucidate the phylogenetic relationships between Sorghum species and ascertain the taxonomy status of the quarantine weeds which provide bases for effective utilization of its germ resources on molecular breeding and improvement of crop qualities and thus provide important guidance for port detection. In the present study, total DNA from the seeds of 8 Sorghum species (four S. almum, two S. halepense, one S. silk and one S. su-danense)were extracted, and the partial Adh1 gene of about 2000 bp in length were amplified by PCR using specific primers designed from conserved regions of Adh1 gene reported in the GenBank (AF050456) and sequenced. Based on these se-quences and other 24 Adh1 sequences registered in the GenBank, the phylogenetic trees constructed by multiple methods (MP, ML, and NJ) with the corresponding Adh1 sequence of Cleistachne sorghoides as the outgroup shared almost the same topology. The results showed that: (1) there were obviously three lineages for the genus Sorghum. One included two sub-genera Chaetosorghum and Heterosorghum, and another included subgenus Eusorghum, both of which consisted of 2n=20 and 2n=40 species with small chromosomes. Still another lineage contained the two subgenera Parasorghum and Stiposor-ghum with 2n=10 species and their polyploid relatives with relatively large chromosomes; (2) geographical divergence clearly existed in the S. almum; (3) S. purpureosericeum, S. versicolor, S. nitidum and S. leiocladum of subgenus Parasor-ghum clustered together, but S. matarankense, S. grande, and S. timorense of the same subgenus clustered with the species of subgenus Stiposorghum, showing closer relationship with subgenus Stiposorghum; (4) S. macrospermum was more closely related to S. laxiflorum than the other species of the genus Sorghum.