遗传 ›› 2009, Vol. 31 ›› Issue (5): 515-515―522.doi: 10.3724/SP.J.1005.2009.00515

• 研究报告 • 上一篇    下一篇

大口黑鲈生长性状的微卫星DNA标记筛选

樊佳佳1,2;白俊杰1;李小慧1;何小平1;何小燕1, 3;李胜杰1;叶星1;吴立新2   

  1. 1. 中国水产科学研究院珠江水产研究所, 中国水产科学研究院热带亚热带鱼类选育与养殖重点开放实验室, 广州 510380;
    2. 大连水产学院生命科学与技术学院, 大连 116023;
    3. 西北农林科技大学动物科技学院, 杨凌 712100
  • 收稿日期:2008-10-16 修回日期:2008-12-22 出版日期:2009-05-10 发布日期:2009-05-10
  • 通讯作者: 白俊杰

Identification of microsatellite markers associated with growth traits in largemouth bass (Micropterus salmoides L.)

FAN Jia-Jia1, 2;BAI Jun-Jie1;LI Xiao-Hui1;HE Xiao-Ping1;HE Xiao-Yan1, 3;LI Sheng-Jie1;YE Xing1;WU Li-Xin2

  

  1. 1. Key Laboratory of Tropical & Subtropical Fish Breeding & Cultivation of Chinese Academy of Fishery Sciences, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China;
    2. School of Life Science and Technology, Dalian Fisheris University, Dalian 116023, China;
    3. College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China
  • Received:2008-10-16 Revised:2008-12-22 Online:2009-05-10 Published:2009-05-10
  • Contact: BAI Jun-Jie

摘要: 本研究在人工养殖的大口黑鲈(Micropterus salmoides L.)群体中对40个微卫星位点进行扩增, 运用卡方检验分析微卫星位点在极端大个体组和极端小个体组中的基因型分布差异, 选择差异显著的16个微卫星位点对大口黑鲈随机群体进行基因型与性状的关联分析, 同时分析群体的遗传多样性。结果表明: 关联分析得到7个微卫星位点(JZL60、JZL67、JZL72、JZL124、MiSaTPW76、MiSaTPW117MiSaTPW173)与体重、体长和体高显著或极显著相关(P<0.05或P<0.01), 同时对差异显著的位点进行不同基因型间与生长性状的多重比较, 找到了与体重、体长和体高性状相关的最有利基因型为JZL60位点的AA、JZL67位点的BB、JZL72位点的AC、MiSaTPW76位点的BBMiSaTPW117位点的BC。应用这16个微卫星位点对随机群体进行遗传多样性分析, 共检测到47个等位基因,平均等位基因2.938个,每个位点检测到的等位基因数为2~5个、群体的平均观测杂合度、平均期望杂合度和平均多态信息含量分别为0.515、0.500和0.445, 表明该群体遗传多样性处于中等水平。

关键词: 大口黑鲈, 微卫星, 生长性状, 关联分析, 遗传多样性

Abstract: Forty microsatellite loci were selected to amplify the genomic DNA of cultured stock of largemouth bass (Micropterus salmoides L.) in China. Genotypic differences of these microsatellites loci were analyzed using c2-test be-tween the maximal weight group and minimal weight group. Sixteen microsatellites showing significantly difference (P<0.1) were used in genotyping 121 individuals, and the associations between their genotypes and growth traits were examined. Microsatellite loci of JZL60, JZL67, JZL72, JZL124, MiSaTPW76, MiSaTPW117 and MiSaTPW173 were significantly as-sociated with body weight, body length, and body height (P<0.05 or P<0.01). The most favorable genotypes for growth traits were AA at JZL60, BB at JZL67, AC at JZL72, BB at MiSaTPW76 and BC at MiSaTPW117. In addition, a total of 47 alleles for the 16 loci were detected (2~5 alleles for each locus). The average effective number of alleles (Ae), observed heterozygosity (Ho), expected heterozygosity (He) and mean polymorphic information content (PIC) was 2.938, 0.515, 0.500 and 0.445, respectively, both of which indicated that the population genetic diversity was medium.