遗传 ›› 2017, Vol. 39 ›› Issue (1): 75-86.doi: 10.16288/j.yczz.16-391

• 资源与平台 • 上一篇    

中华民族永生细胞库在生命科学研究中的支撑作用

许崇凤(),段子渊()   

  1. 中国科学院遗传与发育生物学研究所,北京 100101
  • 收稿日期:2016-11-21 修回日期:2016-12-27 出版日期:2017-01-20 发布日期:2017-12-24
  • 作者简介:许崇凤,博士,助理研究员,研究方向：EBV相关疾病研究。E-mail: cfxu@genetics.ac.cn|段子渊，博士，研究员，研究方向：遗传与进化。E-mail: zyduan@genetics.ac.cn
  • 基金资助:
    中国科学院战略生物资源支撑体系运行专项(CZBZX-1)

A supporting role of Chinese National Immortalized Cell Bank in life science research

Chongfeng Xu(),Ziyuan Duan()   

  1. Institute of Genetics and Developmental biology, Chinese Academy of Sciences，Beijing 100101, China
  • Received:2016-11-21 Revised:2016-12-27 Online:2017-01-20 Published:2017-12-24
  • Supported by:
    Strategic Biological Resources Support System(CZBZX-1)

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摘要：

人类样品是生物医学研究必需的物质基础。B淋巴母细胞系(LCL)是利用Epstein-Barr(EB)病毒转化人的B细胞获得,制备简便,可以无限繁殖,是非常便捷的保存人类样品的形式。中华民族永生细胞库保藏有中国各个民族群体的LCL。目前,已经有详实的LCL的性质研究以及关于LCL的全基因组数据,因而, LCL已经广泛应用于遗传学、免疫学、药学基因组学、再生医学、癌症发生与免疫治疗、筛选制备全人单克隆中和抗体及EB病毒致病机理等研究领域。本文对LCL的特性以及LCL在上述研究领域中的应用进行了综述,最后对中华民族永生细胞库的保藏内容做了简单介绍,以促进广大科研人员进一步了解该细胞库的科研价值,充分发挥该库保藏资源在基础科学、生物医学研究中的科技支撑作用。

关键词: 中华民族永生细胞库, LCL, 药物基因组学, 再生医学, 癌症免疫治疗

Abstract:

A biorepository of human samples is essential to support the research of life science. Lymphoblastoid B cell line (LCL), which is easy to be prepared and can reproduce indefinitely, is a convenient form of sample preservation. LCLs are established from human B cells transformed by Epstein-Barr virus (EBV). Chinese National Immortalized Cell Bank has preserved human LCLs from different ethnic groups in China. As there are many studies on the nature of LCLs and public available resources with genome-wide data for LCLs, they have been widely applied in genetics, immunology, pharmacogenetics/genomics, regenerative medicine, cancer pathogenesis and immunotherapy, screening and generation of fully human neutralizing monoclonal antibodies and study on EBV pathogenesis. Here, we review the characteristics of LCLs and their contributions to scientific research, and introduce preserved samples in Chinese National Immortalized Cell Bank to the scientific community. We hope this bank can support more areas in the scientific research.

Key words: Chinese National Immortalized Cell Bank, LCL, pharmacogenomics, regenerative medicine, cancer immunotherapy