遗传 ›› 2010, Vol. 32 ›› Issue (6): 577-582.doi: 0.3724/SP.J.1005.2010.00577

• 研究报告 • 上一篇    下一篇

云南傣族、汉族HLA-G基因14 bp插入/缺失多态性研究

陈金宝1, 2, 史磊1, 姚宇峰1, 史荔1, 于亮1, 林克勤1, 陶玉芬1, 易文1, 黄小琴1, 孙浩1, 褚嘉祐1   

  1. 1. 中国医学科学院, 北京协和医学院医学生物学研究所遗传室, 昆明650118; 
    2. 昆明医学院, 昆明650031
  • 收稿日期:2009-11-03 修回日期:2009-12-29 出版日期:2010-06-20 发布日期:2010-05-24
  • 通讯作者: 褚嘉祐 E-mail:chujy@imbcams.com.cn
  • 基金资助:

    云南省应用基础研究重点项目(编号:2008CC021)和美国中华医学基金会资助项目(编号:04-805)资助

Analysis of the 14 bp insertion/deletion polymorphism in human leukocyte antigen-G gene in Chinese Dai and Han ethnic groups in Yunnan Province

CHEN Jin-Bao1, 2, SHI Lei1, YAO Yu-Feng1, SHI Li1, YU Liang1, LIN Ke-Qin1, TAO Yu-Fen1, YI Wen1,
 HUANG Xiao-Qin1, SUN Hao1, CHU Jia-You1   

  1. 1. Department of Medical Genetics, Institute of Medical Biology, Chinese Acadamy of Medical Sciences & Peking Union Medical College, Kunming 650118, China;
     2. Kunming Medical College, Kunming 650031, China
  • Received:2009-11-03 Revised:2009-12-29 Online:2010-06-20 Published:2010-05-24
  • Contact: CHU Jia-You E-mail:chujy@imbcams.com.cn

摘要:

14 bp插入/缺失多态性是指存在于HLA-G基因第8外显子3′非翻译区(3′UTR)的一个插入/缺失多态, 因其能影响HLA-G mRNA的稳定性, 并进一步影响HLA-G蛋白的翻译而受到广泛关注。文章采用PCR和电泳技术对云南傣族和汉族两个人群进行了HLA-G基因14 bp插入/缺失多态性的检测分析, 结果显示傣族和汉族人群+14 bp等位基因频率分别为31.97%和40.87%, +14 bp/+14 bp基因型频率分别为8.20%和17.31%, +14 bp/-14 bp基因型频率分别为47.54% 和47.11%。与国内外已报道的其他人群的数据比较表明: 云南汉族群体中HLA-G基因14 bp插入/缺失的分布与其他群体相似; 傣族则有自己独特的基因型和等位基因分布特点, 推测其可能受到了遗传漂变的作用, 但不排除自然选择作用的影响。

关键词: 随机遗传漂变, 自然选择, HLA-G基因, 14 bp插入/缺失多态性

Abstract:

A 14 bp insertion/deletion polymorphism (+14 bp/-14 bp) in exon 8 of HLA-G gene has been investigated extensively because this polymorphism was associated with HLA-G mRNA stability and could influence HLA-G mRNA expression. In the present study, we genotyped 14 bp insertion/deletion polymorphisms in the HLA-G gene in Dai and Han populations in Yunnan Province. The frequencies of +14 bp in Dai and Han were 31.97% and 40.87%, respectively. The frequency of +14 bp/+14 bp in Dai was lower (8.20%) than that of Han (17.31%). Nevertheless, the frequency of +14 bp/-14 bp was similar in both populations (47.54% in Dai and 47.11% in Han). When compared with other populations, the frequency of 14 bp insertion/deletion of Han was similar to others, whereas the Dai showed significent difference from others and presented it’s specific characteristic. We assumed that the distributaion characteristic of 14 bp insertion/deletion in Dai might be the results of random genetic drift, but the role of natural selection cannot be excluded.

Key words: HLA-G gene, 14 bp insertion/deletion polymorphism, random genetic drift, natural selection