杨树MIR169基因家族分子进化分析

doi:10.3724/SP.J.1005.2013.01307

遗传 ›› 2013, Vol. 35 ›› Issue (11): 1307-1316.doi: 10.3724/SP.J.1005.2013.01307

杨树MIR169基因家族分子进化分析

刘志祥^{1, 2, 3,} 曾超珍¹, 谭晓风^{2, 3}

1. 中南林业科技大学生命科学与技术学院, 长沙 410004;
2. 中南林业科技大学林学院, 长沙 410004;
3. 经济林育种与栽培国家林业局重点实验室, 长沙 410004

收稿日期:2013-05-21 修回日期:2013-07-03 出版日期:2013-11-20 发布日期:2013-10-25
通讯作者: 谭晓风, 博士, 教授, 研究方向：林业生物技术。 E-mail:nwfp@foxmail.com
作者简介:刘志祥, 博士研究生, 讲师, 研究方向：植物分子遗传学。Tel: 0731-85623486; E-mail: liuzx@csuft.edu.cn
基金资助:
湖南省自然科学基金项目(编号：13JJ3095)和中南林业科技大学青年基金项目(编号：QJ2011042B)资助

Molecular evolution of the poplar MIR169 gene family

LIU Zhi-Xiang^1,2,3, ZENG Chao-Zhen¹, TAN Xiao-Feng^2,3

1. College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China;
2. College of Forestry, Central South University of Forestry and Technology, Changsha 410004, China;
3. Key Lab of Non-Wood Forest Products of State Forestry Administration, Changsha 410004, China

Received:2013-05-21 Revised:2013-07-03 Online:2013-11-20 Published:2013-10-25
Contact: TAN Xiao-Feng E-mail:nwfp@foxmail.com

摘要/Abstract

摘要：

MicroRNA(miRNA)是真核生物中普遍存在的一类参与基因表达调控的小分子RNA。ptc-MIR169基因家族有33个成员, 是杨树中规模最大的miRNA基因家族。研究MIR169基因家族的进化对揭示杨树miRNA基因的进化机制具有重要的意义。文章对毛果杨MIR169基因家族的分子系统发育、基因倍增模式、表达分化和靶基因进行了分析。结果表明, 染色体大片段重复和串联重复在毛果杨MIR169基因家族扩张中均具有重要作用; MIR169基因家族在表达方式上已经出现了较大的分化; MIR169基因家族可能在杨树中已经形成了复杂的调控网络, 对杨树的生长发育和适应性等具有重要的调控作用。文章为杨树及杨柳科相关植物中miRNA基因家族的分子进化研究提供了参考。

关键词: 毛果杨, MIR169基因家族, 分子进化

Abstract:

MicroRNAs (miRNAs) are a class of short non-coding RNAs found widely in eukaryotic organisms. The ptc-MIR169 gene family, which consists of 33 members, is the largest miRNA gene family in poplar (Populus trichocarpa). It is significant to analyze the evolution of the ptc-MIR169 gene family in order to understand the evolutionary mechanisms of miRNAs in poplar. In the present study, we investigated the molecular phylogeny, duplication, expression and target genes of the MIR169 gene family in poplar. Both tandem duplications and chromosome segmental duplications contributed to the expanding of ptc-MIR169 gene family, and the expression patterns diversified obviously among the gene family. These findings suggest that the ptc-MIR169 gene family is involved in complex regulatory networks, and plays significant roles in development and stress response in poplar. This paper provides a reference for the evolutionary study of miRNAs in poplar and related species in Salicaceae.

Key words: Populus trichocarpa, MIR169 gene family, molecular evolution

刘志祥曾超珍谭晓风. 杨树MIR169基因家族分子进化分析[J]. 遗传, 2013, 35(11): 1307-1316.

LIU Zhi-Xiang ZENG Chao-Zhen TAN Xiao-Feng. Molecular evolution of the poplar MIR169 gene family[J]. HEREDITAS, 2013, 35(11): 1307-1316.

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杨树MIR169基因家族分子进化分析

Molecular evolution of the poplar MIR169 gene family

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