遗传 ›› 2014, Vol. 36 ›› Issue (10): 1043-1052.doi: 10.3724/SP.J.1005.2014.1043

• 研究报告 • 上一篇    下一篇

番茄热激蛋白90的全基因组鉴定及分析

刘云飞1,2,万红建1,杨悦俭1,韦艳萍1,2,李志邈1,叶青静1,王荣青1,阮美颖1,姚祝平1,周国治1   

  1. 1. 浙江省农业科学院蔬菜研究所,杭州 310021;
    2. 南京农业大学园艺学院,南京 210095
  • 收稿日期:2014-02-24 出版日期:2014-10-20 发布日期:2014-10-20
  • 通讯作者: 万红建,博士,副研究员,研究方向:番茄遗传育种。E-mail: wanhongjian@sina.com E-mail:head_20074152@126.com
  • 作者简介:刘云飞,硕士研究生,专业方向:蔬菜学。Tel: 0571-86404352;E-mail: head_20074152@126.com
  • 基金资助:
    国家自然科学基金项目(编号:31301774,31272156),浙江省优先主题农业项目(编号:2011C12004),国家大宗蔬菜产业技术体系(编号:CARS-25-G-16),浙江省农业新品种选育重大科技专项(编号:2012C12903),公益性行业(农业)科研专项经费(编号:201003065),国家科技支撑计划项目(编号:2012BAD02B02)和浙江省蔬菜产业创新团队项目(编号:2009R50026)资助

Genome-wide identification and analysis of heat shock protein 90 in tomato

Yunfei Liu1, 2, Hongjian Wan1, Yuejian Yang1, Yanping Wei1, 2, Zhimiao Li1, Qingjing Ye1, Rongqing Wang1, Meiying Ruan1, Zhuping Yao1, Guozhi Zhou1   

  1. 1. Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
    2. College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2014-02-24 Online:2014-10-20 Published:2014-10-20

摘要: 热激蛋白90(Heat shock protein 90,Hsp90)是植物应对不良环境胁迫产生的一类特定的抗逆蛋白。文章以番茄(Solanum lycopersicum L.)基因组数据为平台,借助生物信息学方法对Hsp90基因家族进行鉴定与分析。结果表明,番茄至少含有7个Hsp90基因,不均匀分布在6条染色体上,氨基酸序列长度为267~794aa,内含子数目为2~19;共线性分析发现两对基因(Hsp90-1Hsp90-3,Hsp90-5Hsp90-7)以片段重复形式存在。MEME(Multiple Em for Motif Elicitation)分析显示,番茄Hsp90基因编码的氨基酸序列具有多个保守基序;聚类分析揭示番茄、水稻(Oryza sativa L.)和拟南芥(Arabidopsis thaliana L.)Hsp90基因可以分为5组,存在3对直系同源基因和4对旁系同源基因;基于RNA-seq数据库表达分析发现,3个基因(Hsp90-5Hsp90-6Hsp90-7)在营养器官和生殖器官中表达量较高,4个基因(Hsp90-1Hsp90-2Hsp90-3Hsp90-4)除在番茄转色后10 d的果实中表达量较高外,其余组织中表达量均较低;对Hsp90基因启动子序列进行分析,发现了多个参与植物对逆境胁迫的顺式作用元件,如HSE、CCAAT-box。此外,qRT-PCR检测结果表明,在叶片热胁迫条件下,番茄Hsp90基因的表达量均存在增强趋势,表明这些基因参与了番茄叶片应对高温胁迫的反应。研究结果为鉴定番茄Hsp90基因的功能和进化起源奠定了基础。

关键词: 番茄, 热激蛋白, 基因重复, 表达分析

Abstract: Heat shock proteins 90 (Hsp90) are a kind of specific proteins in plant which were produced under environmental stresses. By referring to the tomato genome database, we identified and analyzed Hsp90 gene family members using bioinformatics methods. Results indicated that the tomato genome contained at least 7 Hsp90 genes, which were distributed unevenly on 6 chromosomes. Amino acid sequence length of these proteins ranged from 267 to 794aa. Numbers of intron ranged from 2 to 19. Microsynteny analysis showed that two pairs of Hsp90 genes (Hsp90-1and Hsp90-3, Hsp90-5 and Hsp90-7) were identified by segment duplication. In addition, multiple conservation motifs were found in Hsp90 proteins. Phylogenetic analysis revealed that Hsp90 genes from tomato, rice and Arabidopsis can be divided into 5 groups. Three pair of orthologous genes and four pairs of homologous genes were found. Expression analysis based on RNA-seq showed that the expression of three genes (Hsp90-5, Hsp90-6 and Hsp90-7) was high in vegetable and reproductive organs, while the expression of other four genes (Hsp90-1, Hsp90-2, Hsp90-3 and Hsp90-4) was relatively low except for its expression at the breaking stage of fruit. Analysis of promoter regions of Hsp90 genes showed that multiple cis-elements were involved in plant responses to biotic and abiotic stresses. The expression of 7 genes under heat stress was also detected by qRT-PCR. Expression of all Hsp90 genes in tomato leaf was enhanced. The results indicated that these genes could be participated in tomato leaf response to heat stresses. Together, these results will lay a foundation for analyzing Hsp90 gene function and molecular evolution in the future.

Key words: tomato, heat shock protein, gene duplication, expression analysis