雷蒙德氏棉和亚洲棉基因组数据中LPAAT基因家族的发掘及其同源基因在陆地棉材料中的表达分析

doi:10.16288/j.yczz.15-010

遗传 ›› 2015, Vol. 37 ›› Issue (7): 692-701.doi: 10.16288/j.yczz.15-010

雷蒙德氏棉和亚洲棉基因组数据中LPAAT基因家族的发掘及其同源基因在陆地棉材料中的表达分析

马建江¹, 王诺菡^{1, 2}, 吴嫚¹, 裴文锋¹, 王文魁¹, 李兴丽¹, 张金发¹, 喻树迅^{1, 2}, 于霁雯¹

1. 中国农业科学院棉花研究所,棉花生物学国家重点实验室,安阳 455000;
2. 西北农林科技大学农学院,杨凌 712100

收稿日期:2015-01-05 修回日期:2015-05-14 出版日期:2015-07-20 发布日期:2015-07-20
通讯作者: 于霁雯,副研究员,研究方向：棉花遗传育种。E-mail: yujw@cricaas.com.cn
作者简介:马建江,在读硕士研究生,研究方向：棉花遗传育种。E-mail: 504569362@qq.com
基金资助:
国家高技术研究发展计划项目(863计划)(编号2013AA102601-01-14)和国家自然科学基金项目(编号31301368)资助

Genome-wide analysis of the LPAAT gene family in Gossypium raimondii and G. arboreum, and expression analysis of its orthologs in G. hirsutum

Jianjiang Ma¹, Nuohan Wang^{1, 2}, Man Wu¹, Wenfeng Pei¹, Wenkui Wang¹, Xingli Li¹, Jinfa Zhang¹, Shuxun Yu^{1, 2}, Jiwen Yu¹

1. State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China;
2. College of Agronomy, Northwest A&F University, Yangling 712100, China

Received:2015-01-05 Revised:2015-05-14 Online:2015-07-20 Published:2015-07-20

摘要/Abstract

摘要： 溶血磷脂酸酰基转移酶(Lysophosphatidic acid acyltransferase, LPAAT)是油脂合成途径中的一个关键酶,能催化溶血磷脂酸转变为磷脂酸。本研究从雷蒙德氏棉(G. raimondii, D5)和亚洲棉(G. arboreum, A2)的基因组数据中得到17个LPAAT基因家族成员。利用生物信息学方法对二倍体棉花LPAAT基因进行基因结构、染色体分布以及系统进化分析。结果表明,LPAAT基因家族根据亲缘关系的远近可以分为不同的亚家族,各亚家族中LPAAT基因具有相似的基因结构;LPAAT家族基因编码的氨基酸序列具有3个保守基序,其中包括ΦFPEGTR-G结合位点和Φ-NHQS-ΦDΦΦ催化位点;通过对不同物种的LPAAT基因家族进行系统进化分析可知,不同物种中的LPAAT在进化中存在较大差异。基于陆地棉(G. hirsutum)不同发育时期的胚珠RNA-seq数据库和qRT-PCR表达分析,发现LPAAT基因可能对脂肪积累起到积极作用。本研究结果有助于了解棉属植物LPAAT基因家族的功能,以期从中选取较好的LPAAT基因进行进一步功能验证。

关键词: 油脂, LPAAT家族, 基因结构, 系统进化分析, 表达分析

Abstract: Lysophosphatidic acid acyltransferase (LPAAT) which converts lysophosphatidic acid into phosphatidic acid is a key enzyme in biosynthesis pathway of lipid in plants. In this study, we identified 17 members of the LPAAT gene family from genomic data of G. raimondii-D₅ and G. arboreum-A₂. Analysis of gene structure, chromosome distribution and phylogenetic evolution of LPAAT genes in diploid Gossypium using bioinformatics approaches showed that these genes can be divided into distinct subfamilies based on the distance of their genetic relationship. Moreover, the gene structures were similar within LPAAT subfamily members. The amino acid sequences encoded by LPAAT family genes contained three conserved motifs, including ΦFPEGTR-G binding site and Φ-NHQS- ΦDΦΦ catalytic site. Phylogenetic analysis of LPAAT gene family demonstrated significant differences in evolution of LPAAT in different species. Finally, expression analysis of G. hirsutum ovules in different stages from RNA-seq and qRT-PCR data indicated that LPAAT gene may play a positive role in oil accumulation. Our studies facilitate understanding of the function of LPAAT gene family in Gossypium and selecting better LPAAT genes for further functional validation.

Key words: cottonseed oil, LPAAT family, gene structure, phylogenic analysis, expression analysis

马建江, 王诺菡, 吴嫚, 裴文锋, 王文魁, 李兴丽, 张金发, 喻树迅, 于霁雯. 雷蒙德氏棉和亚洲棉基因组数据中LPAAT基因家族的发掘及其同源基因在陆地棉材料中的表达分析[J]. 遗传, 2015, 37(7): 692-701.

Jianjiang Ma, Nuohan Wang, Man Wu, Wenfeng Pei, Wenkui Wang, Xingli Li, Jinfa Zhang, Shuxun Yu, Jiwen Yu. Genome-wide analysis of the LPAAT gene family in Gossypium raimondii and G. arboreum, and expression analysis of its orthologs in G. hirsutum[J]. HEREDITAS(Beijing), 2015, 37(7): 692-701.

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雷蒙德氏棉和亚洲棉基因组数据中LPAAT基因家族的发掘及其同源基因在陆地棉材料中的表达分析

Genome-wide analysis of the LPAAT gene family in Gossypium raimondii and G. arboreum, and expression analysis of its orthologs in G. hirsutum

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