高粱SBP-box基因家族全基因组鉴定及表达分析

doi:10.16288/j.yczz.16-008

遗传 ›› 2016, Vol. 38 ›› Issue (6): 569-580.doi: 10.16288/j.yczz.16-008

高粱SBP-box基因家族全基因组鉴定及表达分析

常建忠¹, 闫凤霞², 乔麟轶³, 郑军³, 张福耀², 柳青山²

1. 山西省农业科学院旱地农业研究中心,农业部黄土高原作物基因资源和种质创制重点实验室,太原030031;
2. 山西省农业科学院高粱研究所,高粱遗传与种质创新山西省重点实验室,晋中 030600;
3. 山西省农业科学院作物科学研究所,太原030031

收稿日期:2016-01-17 修回日期:2016-03-28 出版日期:2016-06-20 发布日期:2016-04-19
通讯作者: 柳青山,硕士,研究员,研究方向：高粱种质资源创新。E-mail: 673673@126.com E-mail:yfxcjz@126.com
作者简介:常建忠,博士,助理研究员,研究方向：作物逆境分子生物学。E-mail: cjzyfx@163.com 闫凤霞,硕士,助理研究员,研究方向：高粱种质资源创新。E-mail: yfxcjz@126.com常建忠和闫凤霞为并列第一作者。
基金资助:
现代农业产业技术体系建设专项(编号：CARS-06-01-01),山西省财政支农项目(编号：2014ZYFZ-09),山西省农业科学院重点项目(编号：YZD1401)和山西省农业科学院博士研究基金项目(编号：YBSJJ1407)资助

Genome-wide identification and expression analysis of SBP-box gene family in Sorghum bicolor L.

Jianzhong Chang¹, Fengxia Yan², Linyi Qiao³, Jun Zheng³, Fuyao Zhang², Qingshan Liu²

1. Dryland Agriculture Research Center, Shanxi Academy of Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan 030031, China;
2. Sorghum Institute of Shanxi Academy of Agricultural Sciences/Shanxi Key Laboratory of Sorghum Genetic and Germplasm Innovation, Jinzhong 030600, China;
3. Institute of Crop Science, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China

Received:2016-01-17 Revised:2016-03-28 Online:2016-06-20 Published:2016-04-19
Supported by:
Supported by the Earmarked Fund for Modern Agro-industry Technology Research System (No; CARS-06-01-01), Financial Supporting Agriculture Project in Shanxi Province (No; 2014ZYFZ-09), Key Project of Shanxi Academy of Agricultural Science (No; YZD1401), and Doctoral Science Foundation of Shanxi Academy of Agricultural Science (No; YBSJJ1407)

摘要/Abstract

摘要： SQUAMOSA PROMOTER BINDING PROTEIN box (SBP-box)基因家族编码一类绿色植物特有的转录因子,其功能涉及作物遗传改良的许多方面,如产量、株型、抗逆性等,具有重要的实际应用价值。本研究通过生物信息学方法从高粱(Sorghum bicolor L.)全基因组中鉴定出18个SBP-box基因,分布于9条染色体上,其中8个基因位于基因组重复区域。系统发育分析表明高粱SBP-box基因家族可分为6个亚家族,其中SbSBP12、SbSBP3和SbSBP15分别与玉米ZmLG1、ZmTGA1和ZmUB2/3直系同源。基于RNA-seq数据的表达分析发现高粱SBP-box基因在花序原基中表达量最高,SbSBP9和SbSBP17为花序原基特异表达基因,SbSBP5、SbSBP8和SbSBP18等基因受外源ABA和PEG胁迫上调表达,表明SBP-box基因可能参与高粱对非生物逆境的响应。本研究为高粱SBP-box家族重要基因的克隆提供了参考,相关基因可作为高粱遗传改良的候选基因。

关键词: 高粱, SBP-box基因家族, 系统进化分析, 表达分析

Abstract: SQUAMOSA PROMOTER BINDING PROTEIN-box (SBP-box) family genes encoding plant-specific transcription factors are involved in many aspects of crop genetic improvement such as yield, plant-type and stress-resistance. The SBP-box gene family have important practical applications. In this study, 18 SBP-box genes were identified from the reference genome of sorghum (Sorghum bicolor L.) using bioinformatics. These genes distributed on nine chromosomes while eight of them located in the segmental duplication region. Phylogenetic reconstruction resulted in six subfamilies of SBP-box genes, among which SbSBP12, SbSBP3 and SbSBP15 are orthologous to ZmLG1, ZmTGA1 and ZmUB2/3 in corn, respectively. RNA-seq data analysis indicated that SbSBP-box genes show the highest expression level in primordial inflorescences. Moreover, SbSBP9 and SbSBP17 exhibited a tissue specific expression in primordial inflorescences. The expression levels of SbSBP5, SbSBP8 and SbSBP18 were increased in response to exogenous ABA and PEG,indicating that SbSBP-box genes are involved in the defense response against abiotic stresses in sorghum. This research provides references for cloning important genes in SbSBP-box gene family. Genes identified in this study could be considered as candidate genes for genetic improvement of sorghum.

Key words: Sorghum bicolor L., SBP-box gene family, phylogenetic analysis, expression analysis

常建忠, 闫凤霞, 乔麟轶, 郑军, 张福耀, 柳青山. 高粱SBP-box基因家族全基因组鉴定及表达分析[J]. 遗传, 2016, 38(6): 569-580.

Jianzhong Chang, Fengxia Yan, Linyi Qiao, Jun Zheng, Fuyao Zhang, Qingshan Liu. Genome-wide identification and expression analysis of SBP-box gene family in Sorghum bicolor L.[J]. HEREDITAS(Beijing), 2016, 38(6): 569-580.

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高粱SBP-box基因家族全基因组鉴定及表达分析

Genome-wide identification and expression analysis of SBP-box gene family in Sorghum bicolor L.

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