FABP4基因在阿勒泰羊尾脂沉积与代谢模型中的表达变化规律

doi:10.16288/j.yczz.14-369

遗传 ›› 2015, Vol. 37 ›› Issue (2): 174-182.doi: 10.16288/j.yczz.14-369

FABP4基因在阿勒泰羊尾脂沉积与代谢模型中的表达变化规律

许瑞霞^1,2,高磊²,赵伟利^1,2,张伟^1,2,宋广超^1,2,甘尚权²,石国庆^1,2

1. 石河子大学动物科技学院,石河子 832000;
2. 新疆农垦科学院畜牧兽医研究所,石河子 832000

收稿日期:2014-10-28 出版日期:2015-02-20 发布日期:2015-01-19
通讯作者: 甘尚权,副研究员,研究方向：分子遗传学。E-mail: shangquangan@163.com
石国庆,研究员,研究方向：动物繁殖与绵羊育种。E-mail: nkkxyxms@163.com
作者简介:许瑞霞,硕士研究生,专业方向：绵羊分子育种设计。Tel: 0993-6683739;E-mail: ruixiaxu2013@163.com
基金资助:
国家自然科学基金新疆联合基金重点项目(编号：U1130302)和“973”前期专项项目(编号：2011CB111501)资助

Analysis of FABP4 expression pattern in rump fat deposition and metabolism of Altay sheep

Ruixia Xu^1,2,Lei Gao²,Weili Zhao^1,2,Wei Zhang^1,2,Guangchao Song^1,2,Shangquan Gan²,Guoqing Shi^1,2

1. College of Animal Science and Technology, Shihezi University, Shihezi 832000, China;
2. Institute of Animal Husbandry and Veterinary, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China

Received:2014-10-28 Online:2015-02-20 Published:2015-01-19

摘要/Abstract

摘要： FABP4(Fatty acid binding protein 4)参与细胞内脂肪酸的转运和脂肪酸代谢,是当前研究动物脂肪沉积与代谢的热门候选基因。为了研究FABP4基因在绵羊尾脂沉积与代谢中的作用,文章采用生物信息学方法分析了FABP4氨基酸序列在各物种中的保守性;利用半定量RT-PCR方法检测了该基因在阿勒泰羊主要组织中的表达;采用饥饿法成功建立了模拟阿勒泰羊尾脂沉积与代谢的动物模型,利用qPCR和iTRAQ(isobaric tags for relative and absolute quantitation)技术同时验证FABP4基因mRNA和蛋白在阿勒泰羊非饥饿组与饥饿组尾脂中的表达变化。序列分析结果表明,绵羊FABP4氨基酸序列在物种间高度保守,提示FABP4基因可能由于其重要的生物功能而在进化中表现出其物种的保守性。组织表达谱结果显示,FABP4 mRNA在阿勒泰羊肠脂与尾脂中均高丰度表达,暗示FABP4基因可能在脂肪中行驶着重要的生理生化功能。qPCR与iTRAQ结果显示,FABP4 mRNA与蛋白在两种极端条件下尾脂中的表达差异均不显著(P>0.05),表明FABP4基因可能不是绵羊尾脂沉积与代谢两种极端差异表型的决定基因。以上研究结果为进一步研究FABP4基因在绵羊尾脂中的生物功能奠定了基础。

关键词: FABP4, 阿勒泰羊, 尾脂沉积与代谢, 表达变化

Abstract: FABP4 (Fatty acid binding protein 4) is a hot candidate gene in fat deposition and lipid metabolism and participates in the transport and metabolism of intracellular free fatty acids. We aim to study the role of FABP4 in fat deposition and metabolism of the rump fat in Altay sheep. In this study, bioinformatics method was used to analyze the protein sequence homology among 10 species, and RT-PCR was employed to detect FABP4 tissue profiling of Altay sheep. An animal model simulating the rump fat deposition and metabolism of Altay sheep was established by continuous starvation, and qPCR and iTRAQ (isobaric tags for relative and absolute quantitation) were used to detecte FABP4 mRNA and protein expression changes in the control and continuous starvation groups, respectively. Sequence analysis showed that FABP4 protein sequence is highly conserved among species, suggesting an important biological function during evolution for FABP4. The RT-PCR result confirmed that FABP4 mRNA was highly expressed in intestinal and rump fat, suggesting that FABP4 plays an important physiological role in fat tissues. We did not find significant differences in FABP4 mRNA and protein between control and continuous starvation groups (P>0.05), which indicates that FABP4 may not be the key gene in fat deposition and metabolism in Altay sheep.The results above lay a foundation for further studies of FABP4 in rump or tail fat.

Key words: FABP4, Altay sheep, rump(tail) fat deposition and metabolism, expression change

许瑞霞,高磊,赵伟利,张伟,宋广超,甘尚权,石国庆. FABP4基因在阿勒泰羊尾脂沉积与代谢模型中的表达变化规律[J]. 遗传, 2015, 37(2): 174-182.

Ruixia Xu,Lei Gao,Weili Zhao,Wei Zhang,Guangchao Song,Shangquan Gan,Guoqing Shi. Analysis of FABP4 expression pattern in rump fat deposition and metabolism of Altay sheep[J]. HEREDITAS(Beijing), 2015, 37(2): 174-182.

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FABP4基因在阿勒泰羊尾脂沉积与代谢模型中的表达变化规律

Analysis of FABP4 expression pattern in rump fat deposition and metabolism of Altay sheep

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