摘要： 建立一个复合扩增体系，同时扩增DYS391、GATA-A4、GATA-A10和GATA-H4 4个Y染色体特异性的STR基因座，并用聚丙烯酰胺凝胶电泳和银染显色技术进行基因分型。采用该复合扩增系统调查广东汉族311名无关男性个体的单倍型频率，这4个基因座分别检出5、7、6和5个等位基因，其基因多样性分别为0.4623、0.6972、0.7173、0.6015。共检出98种单倍型，单倍型基因多样性为0.9755。该复合扩增体系在建立Y染色体STR数据库，研究群体遗传和进行法医学鉴定有重要意义。
Abstract:A multiplex PCR system has been developed to amplify 4 Y-chromosome specific short tandem repeats(STR),DYS391,GATA-A4,GATA-A10 and GATA-H4,simultaneously.PCR products were separated by polyacrylamide gels electrophoresis followed by silver stain.When 311 unrelated males from the Han population in Guangdong were detected by the multiplex system,DYS391,GATA-A4,GATA-A10 and GATA-H4 showed 5,7,6 and 5 alleles respectively.Total 98 haplotypes could be identified.Gene diversity value for the 4 STR was 0.4623,0.6972,0.7173 and 0.6015 respectively.The gene diversity value for the haplotypes of the 4 Y-STR reached 0.9755.The four Y-STR multiplex system will be very powerful for establishing Y-STR database,exploring human origin,paternity testing and personal identification.