湖羊、东湖杂种羊POMC基因外显子3单核苷酸多态性及其与生长性状的关联分析

doi:10.3724/SP.J.1005.2013.01095

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HEREDITAS ›› 2013, Vol. 35 ›› Issue (9): 1095-1100.doi: 10.3724/SP.J.1005.2013.01095

Single nuclear polymorphisms in exon 3 of POMC gene and the asso-ciation with growth traits in Hu sheep and East Friesian × Hu crossbred sheep

WANG Chun-Ling¹, MENG Chen-Ling¹, CAO Shao-Xian², ZHANG Jun², MENG Chun-Hua², WANG Hui-Li², FANG Yong-Fei³, ZHU Dong-Dong⁴, MAO Da-Gan¹

1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
2. Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
3. Shanghai Yonghui Sheep Industry Co., LTD, Shanghai 201808, China;
4. Animal Disease Prevention and Control Center of Jiading District, Shanghai 201800, China

Received:2013-03-30 Revised:2013-06-03 Online:2013-09-20 Published:2013-09-25

Abstract

Abstract:

Pro-opiomelancortin (POMC) plays important roles in the regulation of food intake and energy expenditure. The sheep exon 3 of gene POMC was amplified and sequenced by screening the DNA pools to select single nuclear polymorphisms and analyze the association with the growth traits. Two silent SNP mutations (g.273 T/C and g.456 G/A) in Hu sheep were identified. PCR-restriction fragment length polymorphism (RFLP) was used to test the g.273 T/C and the association between the g.273 T/C polymorphism and some growth traits was analyzed in Hu sheep (n=162) and East Friesian × Hu crossbred sheep (n=130). The results showed that three genotypes, TT, TC and CC, were detected in Hu sheep with the frequencies of 0.469, 0.438 and 0.093, respectively. Two genotypes, TT and TC, were detected in East Friesian × Hu cross-bred sheep with the frequencies of 0.754 and 0.246, respectively. The association analysis showed that in Hu sheep the two-month weaning weight, four-month rump height of genotype CC and the four-month body length, cannon circumfer-ence of genotype TC were significantly higher than those of genotype TT (P<0.05); the four- and six-month weight of geno-type CC were significantly higher than those of genotypes TT and TC (P<0.01); the four-month body height and body length of genotype CC were significantly higher than those of genotypes TT (P<0.01) and TC (P<0.05); the four-month cannon circumference of CC genotype was significantly higher than that of TT genotype (P<0.01). In East Friesian × Hu crossbred sheep the two-month weaning weight, four-month weight, body height, body length, chest depth and cannon cir-cumference of genotype TC were significantly higher than those of genotype TT (P<0.05); the six-month weight of geno-type TC was significantly higher than that of genotype CC (P<0.01). In conclusion, the exon 3 of gene POMC was associ-ated with growth traits, and C allele was beneficial to the increase of body weight and body size traits of sheep, which po-tentially afford a good foundation for further study on POMC gene as aided breeding markers for growth traits in sheep.

Key words: sheep, POMC, PCR-RFLP, growth traits

WANG Chun-Ling MENG Chen-Ling CAO Shao-Xian ZHANG Jun MENG Chun-Hua WANG Hui-Li FANG Yong-Fei ZHU Dong-Dong MAO Da-Gan. Single nuclear polymorphisms in exon 3 of POMC gene and the asso-ciation with growth traits in Hu sheep and East Friesian × Hu crossbred sheep[J]. HEREDITAS, 2013, 35(9): 1095-1100.

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Single nuclear polymorphisms in exon 3 of POMC gene and the asso-ciation with growth traits in Hu sheep and East Friesian × Hu crossbred sheep

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