利用RNA-seq技术筛选大白猪皮下和肌内脂肪 组织差异表达基因

doi:10.16288/j.yczz.17-038

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Hereditas(Beijing) ›› 2017, Vol. 39 ›› Issue (6): 501-511.doi: 10.16288/j.yczz.17-038

• Orginal Article • Previous Articles Next Articles

Identification of differentially expressed genes between subcutaneous and intramuscular adipose tissue of Large White pig using RNA-seq

Wanlong Huang(),Xiuxiu Zhang,Ai Li,Xiangyang Miao()

Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2017-02-09 Revised:2017-03-02 Online:2017-06-20 Published:2017-04-13
Supported by:
the Basic Research Fund for Central Public Research Institutes of CAAS(2013ywf-yb-5, 2013ywf-zd-2);the Agricultural Science and Technology Innovation Program(ASTIP-IAS05);the National High Technology Research Development Program of China (“863” Program)(2008AA10Z140);the Innovation Research Foundation of CAAS(No.2004-CAAS-1);the National Basic Research and Development Program of China (“973” Program)(2015CB943100)

Abstract

Abstract:

To explore the molecular mechanisms of lipid accumulation in different types of adipose tissue, the transcriptomes of the subcutaneous and intramuscular adipose tissues from the Large White pig were determined using RNA-seq technology and bioinformatics methods. The differential gene expression profiles were identified and analyzed with the Gene Ontology, KEGG pathway and protein-protein interaction network strategies. There were 180 differentially expressed genes between the two adipose tissues. The genes up-regulated in the subcutaneous, as compared to intramuscular, adipose tissues were mainly involved in the biological processes related to lipid metabolism. The down-regulated genes were significantly enriched in MAPK signaling pathway, suggesting that this signaling pathway could have an important regulatory role(s) in adipocyte differentiation. In summary, differentially expressed genes between the subcutaneous and intramuscular adipose tissues were predominantly involved in lipid metabolism/accumulation and regulation of adipogenesis in the Large White pig.

Key words: intramuscular fat, subcutaneous fat, lipid metabolism, MAPK signaling pathway, pig, RNA-seq

Wanlong Huang,Xiuxiu Zhang,Ai Li,Xiangyang Miao. Identification of differentially expressed genes between subcutaneous and intramuscular adipose tissue of Large White pig using RNA-seq[J]. Hereditas(Beijing), 2017, 39(6): 501-511.

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