凡纳滨对虾TCP-1-eta基因的克隆及与耐寒性状的相关性

doi:10.3724/SP.J.1005.2011.00168

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HEREDITAS ›› 2011, Vol. 33 ›› Issue (2): 168-174.doi: 10.3724/SP.J.1005.2011.00168

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Molecular cloning of Litopenaeus vannamei TCP-1-eta gene and analysis on its relationship with cold tolerance

YIN Qin^1,2, PENG Jin-Xia¹, CUI Liang¹, XIE Da-Xiang¹, WANG Zhi-Wei², LI Kui², CHEN Xiao-Han¹

1. Guangxi Fishery Institute, Nanning 530021, China;
2. Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2010-06-17 Revised:2010-07-15 Online:2011-02-20 Published:2011-02-25
Contact: CHEN Xiao-Han E-mail:chenxhan@yahoo.com.cn

Abstract

Abstract: To study the molecular mechanism of cold tolerance, we cloned TCP-1-eta homolog gene from Litopenaeus vannamei and studied its relationship with cold tolerance. Based on the sequence from electronic cloning, a pair of primers were designed, and a 1 705 bp cDNA was obtained by RT-PCR. The cDNA contains 1 629 bp ORF, which encodes a peptide of 542 aa. Tissue expression pattern by real-time PCR analysis revealed that TCP-1-eta mRNA mainly existed in muscle tissue. Cold induction by different temperatures revealed that TCP-1-eta mRNA expression started to increase after treatment at 15°C for 36 h, and the increase was remarkable after treatment at 13°C. As for treatment at 13°C for different times, the expression pattern was almost not changed during the first 36 h, but significantly increased after 36 h of treatment. A SNP (single nucleotide polymorphism) site was found in 731 bp of ORF, and the genotypes of 216 Litopenaeus vannamei were determined by PCR-RFLP. Variance analysis indicated that the SNP genotype was significantly related with cold tolerance parameter Cooling-Degree Hours (CDH), and CDH for CC type of Litopenaeus vannamei was longer than TT type.

Key words: Litopenaeus vannamei, TCP-1-eta gene, SNP, cold tolerance

YAN Qi, BANG Jin-Xia, CUI Liang, XIE Da-Xiang, WANG Zhi-Wei, LI Kui, CHEN Xiao-Han. Molecular cloning of Litopenaeus vannamei TCP-1-eta gene and analysis on its relationship with cold tolerance[J]. HEREDITAS, 2011, 33(2): 168-174.

References

[1] Hightower LE. Heat shock, stress proteins, chaperones, and proteotoxicity. Cell, 1991, 66(2): 191-197.

[2] Hendrick JP, Hartl F. Molecular chaperone function of heat-shock proteins. Annu Rev Biohem, 1993, 62: 349-384.

[3] Welch WJ. How cells respond to stress. Sci Am, 1993, 268(5): 56-64.

[4] 程维杰, 李秋玲, 王长法, 王洪梅, 李建斌, 孙延鸣, 仲跻峰. 荷斯坦牛HSP70-1基因遗传多态性与乳腺炎抗性关系. 遗传, 2009, 31(2): 169-174.

[5] Sabahat S, Weiss D, Lurie S. The correlation between heat-shock protein accumulation and persistence and chill-ing tolerance in tomato fruit. Plant Physiol, 1996, 110(2): 531-537. [6] Sato A, Allona I, Collada C, Guevara MA, Casado R, Rodriguez-Cerezo E, Aragoncillo C, Gomez L. Heterologous expression of a plant small heat-shock protein enhances Esherichia coli viability under heat and cold stress. Plant Physiol, 1999, 120(2): 521-528.

[7] Kayukawa T, Chen B, Miyazake S, Shinoda T, Itoyama K, Ishikawa Y. Expression of mRNA for the t-complex polypeptide-1, a subunit of chaperonin CCT, is upregu-lated in association with increased cold hardiness in Delia antique. Cell Stress Chaperon, 2005, 10(3): 204-210.

[8] Campos ÉG, Hamdan FF. Cloning of the chaperonin t-complex polypeptide 1 gene from Schistosoma mansoni and studies of its expression levels under heat shock and oxidative stress. Parasitol Res, 2000, 86(3): 253-258.

[9] Chen ZZ, Cheng CC, Zhang JF, Cao LX, Chen L, Zhou LH, Jin YD, Ye Hua, Deng C, Dai ZH, Xu QH, Hu P, Sun SH, Shen Y, Chen LB. Transcriptomic and genomic evolution under constant cold in Antarctic notothenioid fish. Proc Natl Acad Sci USA, 2008, 105(35): 12944-12949.

[10] 张留所, 相建海.凡纳滨对虾微卫星位点在两个选育家系中遗传的初步研究. 遗传, 2005, 27(6) 919-924.

[11] Livak KJ, Schmittgen TD. Analysis of relative gene expression data using realtime quantitative PCR and the 2-△△CT method. Methods, 2001, 25(4): 402-408.

[12] Chaani A, Gall GAE, Hulata G. Cold tolerance of tilapia species and hybrids. Aquacult Int, 2000, 8(4): 289-298.

[13] Yocum GD, Joplin KH, Denlinger DL. Expression of heat shock proteins in response to high and low temperature extremes in diapausing pharate larve of the gypsy moth, Lymanteia dispar. Arch Insect Biochem Physiol, 1991, 18(4): 239-249.

[14] Airaksinen S, Jokilehto T, Råbergh CMI, Nikinmaa M. Heat-and cold-inducible regulation of HSP70 expression in zebrafish ZF4 cells. Comp Biochem Physiol Part B, 2003, 136(2): 275-282.

[15] Huang LH, Wang CZ, Kang L. Cloning and expression of five heat shock protein genes in relation to cold hardening and development in the leafminer, Liriomyza sativa. J Insect Physiol, 2009, 55(3): 279-285.

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Molecular cloning of Litopenaeus vannamei TCP-1-eta gene and analysis on its relationship with cold tolerance

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