绿色杜氏藻不同β-胡萝卜素羟化酶基因家族胁迫应答模式研究

doi:10.16288/j.yczz.16-327

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Hereditas(Beijing) ›› 2017, Vol. 39 ›› Issue (2): 156-165.doi: 10.16288/j.yczz.16-327

• Original article • Previous Articles Next Articles

Expression analysis of different β-carotenoid hydroxylase gene families in stress response in Dunaliella viridis

Shuaiqi Zhu(),Yifu Gong(),Li Zhang,Kai Yu,Heyu Wang,Xiaojun Yan

School of Marine Sciences, Ningbo University, Ningbo 315211, China

Received:2016-09-22 Revised:2016-12-04 Online:2017-02-17 Published:2017-02-10
Supported by:
Key Scientific and Technological Innovation Team Project of Zhejiang Province Department of Science and Technology(2012R10029-07, 2010R50029);Foundation of Ningbo Science and Technology(2013C10018);the Outstanding (Postgraduate) Dissertation Growth Foundation of Ningbo University(py2014020)

Abstract

Abstract:

β-carotenoid hydroxylase (CHYB) is an important rate-limiting enzyme in the biosynthesis of plant carotenoid. In this study, chyb1 and chyb2, two gene families in Dunaliella viridis were obtained by RNA-seq. The fragment of promoters of CHYB family genes, 1 080 bp for chyb1 (GenBank No. KY012338) and 1 155 bp for chyb2 (GenBank No. KY012339) were cloned by the Genome Walking Technology, respectively. Cis-acting elements of two promoters were analyzed by Plantcare soft. The results show that the chyb1 gene promoter contains more cis-acting elements in responses to abiotic stresses, such as methyl jasmonate, arachidonic acid, acetylsalicylic acid, and so on. On the other hand, the chyb2 promoter contains more cis-acting elements in response to light stress. qRT-PCR results show that the mRNA expression levels of CHYBs are modulated by their promoters, and different CHYB gene families response to distinct stresses.

Key words: Dunaliella viridis, β-carotenoid hydroxylase, gene family, promoter, stress

Shuaiqi Zhu,Yifu Gong,Li Zhang,Kai Yu,Heyu Wang,Xiaojun Yan. Expression analysis of different β-carotenoid hydroxylase gene families in stress response in Dunaliella viridis[J]. Hereditas(Beijing), 2017, 39(2): 156-165.

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Expression analysis of different β-carotenoid hydroxylase gene families in stress response in Dunaliella viridis

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