不同生理年龄毛竹DNA甲基化的MSAP分析

doi:10.3724/SP.J.1005.2011.00794

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HEREDITAS ›› 2011, Vol. 33 ›› Issue (7): 794-800.doi: 10.3724/SP.J.1005.2011.00794

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Research on the features of DNA methylation in leaves of different chronological ages of Phyllostachys heterocycla var. pubescens based on the method of MSAP

GUO Guang-Ping, GU Xiao-Ping, YUAN Jin-Ling, WU Xiao-Li

Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, China

Received:2010-11-25 Revised:2011-03-25 Online:2011-07-20 Published:2011-07-25

Abstract

Abstract: In order to probe the features of DNA methylation for bamboo stand with different chronological ages, the technique of methylation-sensitive amplified polymorphism (MSAP) was employed to detect DNA methylation in the paper. Experiment material is Moso bamboo (Phyllostachys heterocycla var. pubescens) leaves with 3 various chronological ages (5, 31, and >60 years after seed germination). During the procedure of genome DNA extration and MSAP analysis, total 35 pairs of MSAP primers were amplyfied. The results showed that MSAP value for bamboo with those three chrono-logical ages were respectively 24.44%, 28.21% and 32.12%, and full-methylation ratios were 16.57%, 19.41% and 21.23%. Meanwhile, the value of variable sites for methylation reached 52.3% and for demethylation was 10.3%. Therefore, it could be concluded that with ages increasing MSAP value rising for Moso bamboo. Moreover the result of variance analysis for methylation ratio indicated that no significant (P=0.307 > 0.05) difference among individuals with the same ages, while significant (P < 0.001) difference exsited among different chronological ages. Throuygh ANOVA it showed that 6 pairs (E3/HM2, E3/HM6, E3/HM7, E4/HM5, E4/HM6and E5/HM5) of primers had obvious influence on DNA methylation for ones with different chronological ages and could be used for further research.

Key words: Phyllostachys heterocycla var. pubescens, chronological age, DNA methylation, MSAP

GUO An-Beng, GU Xiao-Beng, YUAN Jin-Ling, TUN Xiao-Li. Research on the features of DNA methylation in leaves of different chronological ages of Phyllostachys heterocycla var. pubescens based on the method of MSAP[J]. HEREDITAS, 2011, 33(7): 794-800.

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Research on the features of DNA methylation in leaves of different chronological ages of Phyllostachys heterocycla var. pubescens based on the method of MSAP

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