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HEREDITAS ›› 2011, Vol. 33 ›› Issue (10): 1113-1120.doi: 10.3724/SP.J.1005.2011.01113

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Advances of development of phage display systems

MENG Fan-Mei, ZHANG Chao-Hui, AI Yun-Can   

  1. State Key Lab for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China
  • Received:2011-05-04 Revised:2011-07-31 Online:2011-10-20 Published:2011-10-25

Abstract: The phage display technology (PDT) was unique in genetic engineering and recombinant expression. The phage display systems (PDS) were platforms (kits) composed of genetic modified phages, helper phages, and host bacteria. This review concisely summarized the development of four types of PDS, based on M13, λ, T4, and T7 phages, in terms of phage molecular genetics and genetic (gene or genome) engineering. We addressed on the key components and their genetic (genomic) engineering for modifications, the technical features of different anchors, and the development progress and se-lection reference of those different kits.

Key words: phage, phage display, protein expression, genetic engineering, genomic engineering