2型糖尿病早期大鼠外周神经节基因表达谱分析

doi:10.3724/SP.J.1005.2012.00198

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HEREDITAS ›› 2012, Vol. 34 ›› Issue (2): 198-207.doi: 10.3724/SP.J.1005.2012.00198

Analysis of gene expression profile of peripheral ganglia in early stage type Ⅱ diabetic rats

TANG Xiao-Li^1,2, DENG Li-Bin³, LI Gui-Lin¹, LIU Shuang-Mei¹, LIN Jia-Ri¹, XIE Jin-Yan¹, LIU Jun¹, KONG Fan-Jun¹, LIANG Shang-Dong¹

1. Department of Physiology, Medical College of Nanchang University, Nanchang 330001, China 2. Department of Biochemistry, Medical College of Nanchang University, Nanchang 330001, China 3. Depatment of Cellularbiology, Medical College of Nanchang University, Nanchang 330001, China

Received:2011-05-31 Revised:2011-08-25 Online:2012-02-20 Published:2012-02-25

Abstract

Abstract: Diabetic neuropathy (DN) is defined as the presence of symptoms and/or signs of peripheral nerve dysfunction in people with diabetes. The aim of this study is to screen differentially expressed genes in peripheral ganglia in early stage type Ⅱ experimental diabetic rats. We compared gene expression profiles of peripheral ganglia in type Ⅱ diabetic and nondiabetic rats based on Illumina® Sentrix® BeadChip arrays. The results showed that 158 out of a total of 12 604 known genes were significantly differentially expressed, including 87 up-regulated and 71 down-regulated genes, in diabetic rats compared with those in the nondiabetic rats. It is noted that some up-regulated genes are involved in the biological processes of neuronal cytoskeleton and motor proteins. In contrast, the down-regulated genes are associated with the response to virus\biotic stimulus\ other organism in diabetic rats. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the most significant pathway enriched in the changed gene set is metabolism (P < 0.001). These results indicated that metabolic changes in peripheral ganglia of diabetic rats could be induced by hyperglycemia. Hyperglycemia could change the expression of genes involved in neuronal cytoskeleton and motor proteins through immune inflammatory response, and then impair the structure and function of the peripheral ganglia.

Key words: type 2 diabetes mellitus, peripheral ganglia, gene expression, sprague dawley rats

TANG Xiao-Li, DENG Li-Bin, LI Gui-Lin, LIU Shuang-Mei, LIN Jia-Ri, XIE Jin-Yan, LIU Jun, KONG Fan-Jun, LIANG Shang-Dong. Analysis of gene expression profile of peripheral ganglia in early stage type Ⅱ diabetic rats[J]. HEREDITAS, 2012, 34(2): 198-207.

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