RAD-seq技术在基因组研究中的现状及展望

doi:10.3724/SP.J.1005.2014.00041

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HEREDITAS ›› 2014, Vol. 36 ›› Issue (1): 41-49.doi: 10.3724/SP.J.1005.2014.00041

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Current status and perspective of RAD-seq in genomic research

Yangkun Wang, Yan Hu, Tianzhen Zhang

State Key Laboratory of Crop Genetics and Germplasm Enhancement/Cotton Hybrid R & D Engineering Center of the Ministre of Education, Nanjing Agricultural University, Nanjing 210095, China

Received:2013-07-16 Revised:2013-08-15 Online:2014-01-20 Published:2013-12-20
Contact: Tianzhen Zhang E-mail:cotton@njau.edu.cn
Supported by:
supported by grants from the Major State Basic Research Development Program of China (973 Program)

Abstract

Abstract:

The restriction-site associated DNA sequencing (RAD-seq) is a high-throughput sequencing technique developed from the next-generation sequencing (NGS). This method can reduce the representation of the complex genome while mapping thousands of polymorphic markers with or without a reference genome. It has been extensively used for high-density genetic map construction, fine mapping of important genes, genome sequence assembly, population genomic research, as well as phylogenetic research and so on. Here, we introduce the technological principle and development of RAD-seq combined with the sequencing applications in various species. Due to its uniqueness, RAD-seq will have a wide application in genetic analysis of complex genomic research in the future.

Key words: RAD-seq, genome, genetic map, SNP, double enzyme system of RAD (double digest RAD ddRAD) sequencing

Yangkun Wang, Yan Hu, Tianzhen Zhang. Current status and perspective of RAD-seq in genomic research[J]. HEREDITAS, 2014, 36(1): 41-49.

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