遗传 ›› 2007, Vol. 29 ›› Issue (9): 1126-1130.doi: 10.1360/yc-007-1126

• 研究报告 • 上一篇    下一篇

45S rDNA在小麦及其近缘物种染色体上的分布

徐川梅1, 2, 别同德1, 王春梅1, 周波1, 陈佩度1   

  1. 1.南京农业大学作物遗传与种质创新国家重点实验室,南京 210095;
    2.浙江林学院浙江省现代森林培育技术重点实验室,临安 311300

  • 收稿日期:2007-03-19 修回日期:2007-06-20 出版日期:2007-09-10 发布日期:2007-09-10
  • 通讯作者: 徐川梅

Distribution of 45S rDNA sequence on chromosomes of Triticum aes-tivum and its relative species

XU Chuan-Mei1,2, BIE Tong-De1, WANG Chun-Mei1, ZHOU Bo1, CHEN Pei-Du1

  

  1. 1. National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China;
    2. Key Lab for Modern Silvicultural Technology of Zhejiang Province, Zhejiang Forestry College, Linan 311300, China

  • Received:2007-03-19 Revised:2007-06-20 Online:2007-09-10 Published:2007-09-10
  • Contact: XU Chuan-Mei

摘要:

将染色体C-分带和原位杂交技术相结合,系统研究了45S rDNA在栽培一粒小麦、野生二粒小麦、普通小麦、大麦、簇毛麦、硬簇麦、六倍体燕麦及鹅观草等物种染色体上的分布情况。这些物种染色体的次缢痕区都有45S rDNA位点, 某些非随体染色体上也有45S rDNA位点分布。以小麦—鹅观草1Rk#1二体附加系为材料,通过顺序C分带-FISH技术首次将一个45S rDNA定位到1Rk#1染色体短臂末端。

关键词: 45S rDNA, 顺序C分带-FISH技术, 小麦近缘物种

Abstract:

Fluorescent in situ hybridization (FISH) and sequential C-banding and FISH techniques were used in locating 45S rDNA sequence on specific chromosomes of Triticum monococcum, T. dicoccoides, T. aestivum, Hordeum vulgare, Haynaldia villosa, T. durum-Haynaldia villosa amphiploid, Avena fatus, and Roegneria kamoji. High polymorphism between common wheat and its relatives on 45S rDNA sequence distribution was detected. All the chromosomal secondary constriction regions of these species had 45S rDNA loci. Some non-satellite chromosomes had additional 45S rDNA loci. A 45S rDNA locus was located on the terminal region of the short arm of R. kamoji chromosome 1Rk#1.