高油酸花生发芽期低温胁迫转录组及差异表达基因分析

doi:10.16288/j.yczz.19-097

遗传 ›› 2019, Vol. 41 ›› Issue (11): 1050-1059.doi: 10.16288/j.yczz.19-097

高油酸花生发芽期低温胁迫转录组及差异表达基因分析

张高华¹,于树涛²,王鹤¹,王旭达¹()

^1. 辽宁省海洋水产科学研究院,大连 116023
^2. 辽宁省沙地治理与利用研究所,阜新 123000

收稿日期:2019-04-04 修回日期:2019-09-05 出版日期:2019-11-20 发布日期:2019-10-18
通讯作者: 王旭达 E-mail:wangxuda860@sina.com
作者简介:张高华,博士,研究方向：植物抗逆基因工程。E-mail: zhgaohua@hotmail.com
基金资助:
辽宁省自然科学基金项目资助编号：(20180551263)

Transcriptome profiling of high oleic peanut under low temperatureduring germination

Gaohua Zhang¹,Shutao Yu²,He Wang¹,Xuda Wang¹()

^1. Liaoning Ocean and Fisheries science research Institute, Liaoning Academy of Agricultural Sciences, Dalian116023, China
^2. Sandy Land Amelioration and Utilization Research Institute of Liaoning, Fuxin123000, China

Received:2019-04-04 Revised:2019-09-05 Online:2019-11-20 Published:2019-10-18
Contact: Wang Xuda E-mail:wangxuda860@sina.com
Supported by:
Supported by the Natural Science Foundation of Liaoning Province No.(20180551263)

摘要/Abstract

摘要：

高油酸花生(Arachishypogaea L.)油具有优异的营养成分和热氧化稳定性,有利于人体健康和工业生产。但是高油酸花生在发芽期间对温度比较敏感,限制了其在低温地区的引种。为了进一步了解高油酸花生在发芽期响应低温胁迫的分子机制,本研究选用田间试验中耐寒表现不同的4种高油酸花生品种,分析其在发芽期低温胁迫下的全基因组水平调控。通过转录组高通量测序共获得139 429条Unigene,其中两组耐寒与不耐寒高油酸花生品种在低温胁迫下共产生差异表达基因(differentially expressed gene, DEG) 3520个,且耐寒花生中上调表达的DEG数目大于下调表达的数目。GO分析表明,耐寒高油酸花生中有关细胞膜代谢与完整性以及细胞外周蛋白差异表达基因的数量较多;KEGG通路分析表明,植物病原相互作用和植物激素信号转导通路在抗寒中起着重要作用。进一步筛选出4个低温诱导蛋白基因——时钟调控蛋白基因(TIC) 、AGO4蛋白基因(AGO4)、组蛋白-赖氨酸N甲基类转移酶ATX3基因(ATX3)、FERONIA类受体蛋白激酶基因(FER)和3个转录因子基因——bHLH、3R-1MYB和EREB,采用qRT-PCR检测这些基因在低温胁迫下的表达量。结果显示,在低温处理3 h后TIC、ATX3和AGO4以及转录因子基因bHLH、MYB和EREB的表达量明显升高,FER在胁迫12 h后也有明显升高,表明这些基因在花生萌发期响应低温胁迫。本研究为深入了解高油酸花生发芽期低温胁迫转录调控机制和筛选花生抗寒基因提供了数据资源。

关键词: 花生, 高油酸, 转录组分析, 耐寒

Abstract:

High oleic (HO) peanut (Arachishypogaea L.) oils benefit human health and industrial production due to its superior nutritional composition and thermo-oxidative stability. However, HO peanut is sensitive to cold stress especially during germination, which limits its distribution in low temperature areas. To understand the molecular mechanism of cold responses in HO peanuts at germination stage, four HO peanut varieties with different cold tolerance were selected in field experiments to analyze their genome-wide gene regulation under low temperatures. High-throughput sequencing and transcriptome analysis revealed a total of 139 429 unigenes. Among these, 3520 common differentially expressed genes (DEG) were detected between two groups of cold-tolerant and cold-sensitive peanuts, and the number of up-regulated genes was greater than that of down-regulated genes in the cold-tolerant peanuts. Gene ontology analysis indicates that the number of DEGs involved in cell membrane metabolism and integrity as well as proteins located in the cell periphery were significantly higher in the cold-tolerant peanuts. KEGG pathway analysis suggests that plant-pathogen interaction and plant hormone signal transduction pathway play important roles in cold tolerance. Four cold-induced genes, TIC(TIME FOR COFFEE), ATX3(histone-lysine N-methyltransferase ATX3-like), AGO4(argonaute 4-like), FER(FERONIA-like receptor protein kinase), and three transcription factor genes, bHLH(bHLH49-like transcription factor), MYB(MYB-related protein 3R-1-like)and EREB(Ethylene-responsive element binding factor 6)were selected to verify the expression profile via real-time quantitative PCR detection. The expression of TIC, ATX3, AGO4, bHLH, MYB and EREB significantly increased within 3 hours after low temperature stress, while the expression of FER significantlyincreased after 12 hours, suggesting that these genes responded to low temperature stress during peanut germination. This work not only sheds light on the transcriptional regulation of HO peanut under low-temperature stress during germination but also provides data resources for screening candidate genes in improving peanuts stress resistance.

Key words: peanut, high oleic acid, transcriptome analysis, cold tolerance

张高华, 于树涛, 王鹤, 王旭达. 高油酸花生发芽期低温胁迫转录组及差异表达基因分析[J]. 遗传, 2019, 41(11): 1050-1059.

Gaohua Zhang, Shutao Yu, He Wang, Xuda Wang. Transcriptome profiling of high oleic peanut under low temperatureduring germination[J]. Hereditas(Beijing), 2019, 41(11): 1050-1059.

参考文献 30

[1]	Heidarvand L, Amiri RM . What happens in plant molecular responses to cold stress? Acta Physiologiae Plantarum, 2010,32(3):419-431.
[2]	Conrath U . Molecular aspects of defence priming. Trends Plant Sci, 2011,16(10):524-531.
[3]	Zhang XH, Jaime A, Da Silva T, Niu MY, Li MZ, He CM, Zhao JH, Zeng SQ, Duan J, Ma GH . Physiological and transcriptomic analyses reveal a response mechanism to cold stress in Santalum album L. leaves. Sci Rep, 2017,7:42165.
[4]	Barrero-Gil J, Salinas J . Gene regulatory networks mediating cold acclimation: The CBF pathway. AdvExp Med Biol, 2018,1081:3-22.
[5]	Robison JD, Yamasaki YL, Randall SK . The ethylene signaling pathway negatively impacts CBF/DREB-regulated cold response in soybean ( Glycine max). Front Plant Sci, 2019,10:121.
[6]	Chen J . Advances in genetics and breeding of high oleic acid peanut. J Plant Genet Resour, 2011,12(2):190-196.
	陈静 . 高油酸花生遗传育种研究进展. 植物遗传资源学报, 2011,12(2):190-196.
[7]	Zheng C, Yang M, Zhou Q, Huang FH, Deng QC, Guo PM, Liu CS . Contents of fatty acid and minor component and oxidative stability of high oleic peanut oil and normal oleic peanut oil. China Oil, 2014,39(11):40-43.
	郑畅, 杨湄, 周琦, 黄凤洪, 邓乾春, 郭萍梅, 刘昌盛 . 高油酸花生油与普通油酸花生油的脂肪酸、微量成分含量和氧化稳定性. 中国油脂, 2014,39(11):40-43.
[8]	Chi XY, Chen MN, Pan L, Chen N, Wang T, Wang M, Yang Z, Yu SL . Research progress on high-oleic acid peanut breeding. J PeanSci, 2014,43(4):32-38.
	迟晓元, 陈明娜, 潘丽, 陈娜, 王通, 王冕, 杨珍, 禹山林 . 花生高油酸育种研究进展. 花生学报, 2014,43(4):32-38.
[9]	Barkley NA, Isleib TG, Wang ML, Pittman RN . Genotypic effect of ahFAD2 on fatty acid profiles in six segregating peanut(Arachishypogaea L.)populations. BMC Genet, 2013, 14:62.
[10]	Song JC, Li SZ, Wang JY, Zhang XG, Zhu XF, Qiao JL, Xiang Z . Advances in breeding of high oil peanut in China. Crops, 2018, (3):25-31.
	宋江春, 李拴柱, 王建玉, 张秀阁, 朱雪峰, 乔建礼, 向臻 . 我国高油花生育种研究进展. 作物杂志, 2018, ( 3):25-31.
[11]	Ge LQ, Yan LP, Jia K . A comparative study on scientific research status of peanut industry in Liaoning and Shandong provinces. Agric Econ, 2017, ( 10):12-13.
	葛立群, 闫立萍, 贾可 . 辽宁、山东两省花生产业科研现状对比研究. 农业经济, 2017, ( 10):12-13.
[12]	Zhang MW, Yu GQ, Yu ST, Yu HB, Shi PX . Planting technology of high oleic peanut in liaoning province. AgricEngTechnol, 2018, 38(8):53.
	张明威, 于国庆, 于树涛, 于洪波, 史普想 . 辽宁高油酸花生种植技术. 农业工程技术, 2018,38(8):53.
[13]	Yu ST, Yu GQ, Sun HX, Ren L, Wang H, Zhao LR, Yu HB . Genetic diversity analysis on high oleic acid peanut (Arachishypogaea L.) varieties and strains. Mol Plant Breed, 2017,15(10):4033-4039.
	于树涛, 于国庆, 孙泓希, 任亮, 王虹, 赵立仁, 于洪波 . 高油酸花生品种(品系)的遗传多态性分析. 分子植物育种, 2017,15(10):4033-4039.
[14]	Narsai R, Gouil Q, Secco D, Srivastava A, Karpievitch YV, Liew LC, Lister R, Lewsey MG, Whelan J . Extensive transcriptomic and epigenomicremodelling occurs during Arabidopsis thaliana germination. Genome Biol, 2017,18(1):172.
[15]	Bellieny-Rabelo D, De Oliveira EA, Ribeiro ES, Costa EP, Oliveira AE, Venancio TM . Transcriptome analysis uncovers key regulatory and metabolic aspects of soybean embryonic axes during germination. Sci Rep, 2016,6:36009.
[16]	Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ . TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana. Plant J, 2013,76(2):188-200.
[17]	Müller K, Bouyer D, Schnittger A, Kermode AR . Evolutionarily conserved histone methylation dynamics during seed life-cycle transitions. PLoS One, 2012,7(12):e51532.
[18]	Mallory A, Vaucheret H . Form, function, and regulation of ARGONAUTE proteins. Plant Cell, 2010,22(12):3879-3889.
[19]	Feng W, Kita D, Peaucelle A, Cartwright HN, Doan V, Duan Q, Liu MC, Maman J, Steinhorst L, Schmitz-Thom I, Yvon R, Kudla J, Wu HM, Cheung AY, Dinneny JR . The FERONIA receptor kinase maintains cell-wall integrity during salt stress through Ca 2+signaling . CurrBiol, 2018,28(5):666-675.
[20]	Singh M, Singh J . Seed development-related expression of ARGONAUTE4_9 class of genes in barley: possible role in seed dormancy. Euphytica, 2012,188(1):123-129.
[21]	Singh M, Singh S, Randhawa H, Singh J . Polymorphic homoeolog of key gene of RdDM pathway, ARGONAUTE4_9 class is associated with pre-harvest sprouting in wheat ( Triticumaestivum L.). PLoS One, 2013,8(10):e77009.
[22]	Nonogaki H . Seed dormancy and germination-emerging mechanisms and new hypotheses. Front Plant Sci, 2014,5:233.
[23]	Liu Y, Zhang A, Yin H, Meng Q, Yu X, Huang S, Wang J, Ahmad R, Liu B, Xu ZY . Trithorax-group proteins ARABIDOPSIS TRITHORAX4 (ATX4) and ATX5 function in abscisic acid and dehydration stress responses. New Phytol, 2018,217(4):1582-1597.
[24]	Chen LQ, Luo JH, Cui ZH, Xue M, Wang L, Zhang XY, Pawlowski WP, He Y . ATX3, ATX4, and ATX5 encode putative H3K4 methyltransferases and are critical for plant development. Plant Physiol, 2017,174(3):1795-1806.
[25]	Jin Y, Zhai S, Wang W, Ding X, Guo Z, Bai L, Wang S . Identification of genes from the ICE-CBF-COR pathway under cold stress in Aegilops-Triticum composite group and the evolution analysis with those from Triticeae. Physiol MolBiol Plants, 2018,24(2):211-229.
[26]	Zhang Fu, Jiang Y, Bai LP, Zhang L, Chen LJ, Li HG, Yin Y, Yan WW, Yi Y, Guo ZF . The ICE-CBF-COR pathway in cold acclimation and AFPs in plants. Middle-East J Sci Res, 2011, 8(2):493-498.
[27]	Liu YK, Dang PY, Liu LX, He CZ . Cold acclimation by the CBF-COR pathway in a changing climate: Lessons from Arabidopsis thaliana. Plant Cell Rep, 2019,38(5):511-519.
[28]	Shi YT, Ding YL, Yang SH . Molecular regulation of CBF signaling in cold acclimation. Trends Plant Sci, 2018,23(7):623-637.
[29]	Wang Y, Chen KP, Yao Q . Progress of studies on bHLH transcription factor families. Hereditas(Beijing), 2008,30(7):821-830.
	王勇, 陈克平, 姚勤 . bHLH转录因子家族研究进展. 遗传, 2008,30(7):821-830.
[30]	Liu JY, Shi JY, Yang SH . CBF: A key factor balancing plant cold stress responses and growth. Chin Bull Bot, 2017,52(6):689-698.
	刘静妍, 施怡婷, 杨淑华 . CBF: 平衡植物低温应答与生长发育的关键. 植物学报, 2017,52(6):689-698.

编辑推荐

Metrics

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

高油酸花生发芽期低温胁迫转录组及差异表达基因分析

Transcriptome profiling of high oleic peanut under low temperatureduring germination

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献 30

相关文章 7

编辑推荐

Metrics

[1]	曾瑞儿, 耿庆辉, 高恒宽, 潘晴晴, 陈婷婷, 陈勇, 张雷. 氮素调控花生共生结瘤的机制研究进展[J]. 遗传, 2023, 45(9): 801-812.
[2]	李湘龙，柏斌，吴俊，邓启云，周波. 第二代测序技术用于水稻和稻瘟菌互作早期转录组的分析[J]. 遗传, 2012, 34(1): 102-112.
[3]	彭文舫，吕建伟，任小平，黄莉，赵新燕，文奇根，姜慧芳. 花生抗青枯病相关基因的差异表达[J]. 遗传, 2011, 33(4): 389-396.
[4]	殷勤，彭金霞，崔亮，谢达祥，王志伟，李奎，陈晓汉. 凡纳滨对虾TCP-1-eta基因的克隆及与耐寒性状的相关性[J]. 遗传, 2011, 33(2): 168-174.
[5]	潘星华，朱海英，MARJANI Sadie L.. 单细胞基因组学分析的技术前沿[J]. 遗传, 2011, 33(1): 17-24.
[6]	雷，永，廖伯寿，王圣玉，张银波，李，栋，姜慧芳. 花生黄曲霉侵染抗性的SCAR标记[J]. 遗传, 2006, 28(9): 1107-1111.
[7]	魏东，周俊初. 用电脉冲法将外源质粒DNA导入花生根瘤菌的研究[J]. 遗传, 1996, 18(3): 30-33.