遗传 ›› 1998, Vol. 20 ›› Issue (3): 5-7.

• 论文 • 上一篇    下一篇

雄性不育嵌合基因的构建及番茄转化研究

张宏1; 王波1; 薛爱群1; 曹俊1; 李宝健2; 谭兆平2; 黄伟如2 ZHANG Hong1;WANG Bo1;XUE Ai-qun1;CAO Jun1;LI Bao-jian1;TAN Zhao-ping2;HUANG Wei-ru2   

  1. 1.广州中山大学生物工程研究中心,广州 510275 2.广州市蔬菜科学研究所,广州 510315 1.Biotechnology Research Center,Zhongshan University,Guangzhou 510275 2.Guangzhou Vegetable Scientific Research Institute,Guangzhou 510315
  • 收稿日期:1900-01-01 出版日期:1998-06-10 发布日期:1998-06-10

The Constuction and Tomato Tranformation of Male SterileChimeric Gene

  • Received:1900-01-01 Online:1998-06-10 Published:1998-06-10

摘要: 用从烟草里克隆的TA29启动子分离自Bacillus amyloliquefaciens的核糖核酸酶基因barnase,构建成雄性不育嵌合基因。再用农杆菌介导法转化番茄子叶,获得了具有雄性不育特征的转基因植株。
Abstract:The utilization of heterosis based on male sterility has great significance in raising crop yeld and improving quality.A tapetum-specific promoter TA29 from tobacco young leaves,and a Rnase gene-barnase from Bacillus amyloliquefaciens have been cloned for the construction of male sterile chimeric gene.Transgenic tomato plants with male sterile property have been obtained after transformation with this gene.

关键词: 雄性不育, 转基因, Male sterility, 番茄
Key words,
Transgenic, TH01, TPOX, DP, CSF1PO, 中国汉族, STR

Key words: TPOX, STR, CSF1PO, TH01, Discrimination power(DP), Han Chinese