遗传 ›› 2000, Vol. 22 ›› Issue (2): 86-89.
田长恩1;王正询1;陈韬1;周玉萍1;黄自然2;黄亚东2 TIAN Chang-en1;WANG Zheng-xun1;CHEN Tao1;ZHOU Yu-ping1;HANG Zi-ran2;HANG Ya-dong2
摘要： 用花粉管通道法将柞蚕抗菌肽D基因导入番茄，获得了转基因植株。应用PCR技术、DNA斑点杂交以及Southern印迹分析表明，抗菌肽D基因已整合到番茄基因组；而且，位于目的基因下游的胭脂碱合成酶基因有表达。青枯假单孢菌活体接种结合高发病大田种植试验结果显示，部分转基因植株的子一代具有较强的抗青枯病能力。
Abstract:By using pollen-tube pathway,antibacterial peptide D(AP-D) gene developed from Chinese oak silkwarm was transferred into tomato.PCR,dot and Southern blot analysis confirmed that stable integration of AP-D gene into the tomato genome had occurred.The expression of NOP gene which was localized at downstream of AP-D gene was confirmed with nopaline detection.In comparision with non-transgenicplants,some transgenic plant expressed a stronger resistance value to bacterialwilt disease (Pseudomonas solanacearum).
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