黄瓜叶片细菌性角斑病侵染初期cDNA文库分析

doi:10.3724/SP.J.1005.2009.01042

遗传 ›› 2009, Vol. 31 ›› Issue (10): 1042-1048.doi: 10.3724/SP.J.1005.2009.01042

黄瓜叶片细菌性角斑病侵染初期cDNA文库分析

刘关君^1,2;王丽娟¹;秦智伟¹;孟令波¹

1. 东北农业大学园艺学院, 哈尔滨 150030;
2. 东北林业大学林木遗传育种与生物技术教育部重点实验室, 哈尔滨 150040

收稿日期:2009-03-03 修回日期:2009-05-22 出版日期:2009-10-10 发布日期:2009-10-10
通讯作者: 秦智伟

Analysis of cDNA library of Cucumis sativus L. challenged by Pseu-domonas syringae pv. Lachrymans

LIU Guan-Jun^1,2;WANG Li-Juan¹;QIN Zhi-Wei¹;MENG Ling-Bo¹

1. College of Horticulture, Northeast Agricultural University, Harbin 150030, China; 2. The Laboratory of Forest Genetics and Breeding and Biotechnology of Ministry of Education, Northeast Forestry University, Harbin 150040, China

Received:2009-03-03 Revised:2009-05-22 Online:2009-10-10 Published:2009-10-10
Contact: QIN Zhi-Wei

摘要/Abstract

摘要：

文章以接种细菌性角斑病原菌48 h的抗病品种‘D0462’黄瓜叶片为材料, 构建了黄瓜叶片cDNA文库, 文库插入片段大小为0.45~2.1 kb之间, 平均长度为1 kb。随机选取2 966个克隆进行测序, 共拼接出2 352个假定独立转录本(TUTs), 其中包括282个重叠群(Contigs), 2 070个单拷贝EST(Singlets)。经BlastX分析共获得已知功能和未知功能基因1 848个, 无序列相似性新基因504个。文库中含有大量防御/抗病基因, 如金属硫蛋白、谷胱甘肽-S-转移酶、泛素、b-1, 3-葡聚糖酶、锌指蛋白和半胱氨酸蛋白酶等, 这些基因很可能参与了植物与病原菌互作过程。

关键词: 黄瓜, 叶片, 细菌性角斑病, cDNA文库

Abstract:

A cDNA library was constructed from the leaves of the disease-resistant cucumber (Cucumis sativus L.) cultivar ‘D0462’ challenged by Pseudomonas syringae pv. Lachrymans for 48 h. The inserted fragment sizes ranged from 0.45 to 2.1 kb and the average inserted size was 1 kb. Sequencing analysis showed that 2 352 TUTs (Tentative unique transcripts), 282 contigs, and 2 070 singlets were identified in the 2 966 ESTs derived from the cDNA library. The result of the BlastX analysis indicated that there were 1 848 ESTs with known or unknown function, 504 ESTs with no significant similarity matching with any protein or DNA sequence in the databases. In this library, many defense/disease-resistant related genes, such as metallothionein, glutathione S-transferase, ubiquitin, b-1, 3-glucanase, zinc finger protein, and cysteine protease, which might participate in the plant and the pathogens, are inclued.

刘关君，王丽娟，秦智伟，孟令波 . 黄瓜叶片细菌性角斑病侵染初期cDNA文库分析[J]. 遗传, 2009, 31(10): 1042-1048.

LIU Guan-Jun, WANG Li-Juan, QIN Zhi-Wei, MENG Ling-Bo. Analysis of cDNA library of Cucumis sativus L. challenged by Pseu-domonas syringae pv. Lachrymans[J]. HEREDITAS, 2009, 31(10): 1042-1048.

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黄瓜叶片细菌性角斑病侵染初期cDNA文库分析

Analysis of cDNA library of Cucumis sativus L. challenged by Pseu-domonas syringae pv. Lachrymans

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