安全转基因技术研究进展

doi:10.3724/SP.J.1005.2011.00437

遗传 ›› 2011, Vol. 33 ›› Issue (5): 437-442.doi: 10.3724/SP.J.1005.2011.00437

安全转基因技术研究进展

张茜¹, 张金凤¹, 付文锋¹, 张鸿景², 袁文军³

1. 北京林业大学, 林木育种国家工程实验室, 国家林业局树木花卉育种与生物工程重点开放实验室, 林木、花卉遗传育种教育部重点实验室, 北京 100083 2. 河北省林业科学研究院, 石家庄 050061 3. 河北省围场满族蒙古族自治县林业局, 围场县 068450

收稿日期:2011-03-30 修回日期:2011-04-12 出版日期:2011-05-20 发布日期:2011-05-25
通讯作者: 张金凤 E-mail:zjf@bjfu.edu.cn
基金资助:
国家自然科学基金项目(编号：31070598)和教育部科学技术研究重点项目(编号：109022)资助

Advances on transgene containment technologies

ZHANG Qian¹, ZHANG Jin-Feng¹, FU Wen-Feng1, ZHANG Hong-Jing2, YUAN Wen-Jun3

1. National Engineering Laboratory for Tree Breeding, Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, the Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration, Beijing Forestry University, Beijing 100083, China 2. Hebei Academy of Forestry Science, Shijiazhuang 050061, China 3. Forest Bureau of Weichang Mancu and Mogolia Autonomy County, Weichang 068450, China

Received:2011-03-30 Revised:2011-04-12 Online:2011-05-20 Published:2011-05-25
Contact: ZHANG Jin-Feng E-mail:zjf@bjfu.edu.cn

摘要/Abstract

摘要： 转基因生物安全性问题已引起了公众的普遍担忧, 严重制约了转基因技术成果的推广应用。近年来, 研究者探索了不同的技术策略来解决转基因生物的安全性问题, 安全转基因技术成为当前转基因研究的热点之一。文章介绍并评价了几种主要的安全转基因技术, 包括无选择性标记基因技术、安全标记基因技术、叶绿体转化技术、终止子技术、雄性不育技术、外源基因删除技术。其中, 外源基因删除技术为实现转基因生物的安全应用展示了诱人的前景。最后, 对探索新的安全转基因技术策略保障转基因生物安全提出了建议。

关键词: 转基因生物, 生物安全性, 安全转基因技术, 外源基因删除技术

Abstract: The biosecurity of transgenic organism has been widely concerned and extremely restricted its application. Recently, many technological strategies have been developed to ensure its biosecurity. Thus, transgene containment technologies have become one of the hotspots in current transgenic research. In this paper, several transgene containment technologies, such as marker-free transgenic technology, safety marker transgenic technology, chloroplast transgenic technologies, terminator technology, male sterility technology, and ‘GM-gene-deletor’technology were reviewed and evaluated. ‘GM-gene-deletor’ technology, as one of these technologies, demonstrated a prosperous future for safe application of transgenic organisms. Finally, the strategies for developing new transgene containment technologies have been suggested.

Key words: transgenic organism, biosecurity, transgene containment technologies, ‘GM-gene-deletor’technology

张茜，张金凤，付文锋，张鸿景，袁文军. 安全转基因技术研究进展[J]. 遗传, 2011, 33(5): 437-442.

ZHANG Qian, ZHANG Jin-Feng, FU Wen-Feng, ZHANG Hong-Jing, YUAN Wen-Jun. Advances on transgene containment technologies[J]. HEREDITAS, 2011, 33(5): 437-442.

参考文献

[1] 卢宝荣, 张文驹, 李博. 转基因的逃逸及生态风险. 应用生态学报, 2003, 14(6): 989-994.
[2] 章家恩, 骆世明. 农业生态安全及其生态管理对策探讨. 生态学杂志, 2004, 23(6): 59-62.
[3] Daley M, Knauf VC, Summerfelt KR, Turner JC. Co-transformation with one Agrobacterium tumefaciens strain containing two binary plasmids as a method for producing marker-free transgenic plants. Plant Cell Rep, 1998, 17(6-7): 489-496.
[4] Komari T, Hiei Y, Saito Y, Murai N, Kumashiro T. Vectors carrying two separate T-DNAs for co-transformation for higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers. Plant J, 1996, 10(1): 165-174.
[5] Huang S, Gilbertson LA, Adams TH, Malloy KP, Reisen-bigler EK, Birr DH, Snyder MW, Zhang Q, Luethy MH. Generation of marker-free transgenic maize by regular two border Agrobacterium transformation vectors. Trans Res, 2004, 13(5): 451-461.
[6] Ow DW. Recombinase-directed plant transformation for the post-genomic era. Plant Mol Biol, 2002, 48(1-2): 183-200.
[7] Dale EC, Ow DW. Gene transfer with subsequent removal of the selection gene from the host genome. Proc Natl Acad Sci USA, 1991, 88(23): 10558-10562.
[8] Lloyd AM, Davis RW. Functional expression of the yeast FLP/FRT site-specific recombination system in Nicotiana tabacum. Mol Genet Genomics, 1994, 242(6): 653-657.
[9] Shan XY, Li B, Zhang JR. Production of marker-free transgenic tobacco plants by FLP/frt recombination system. Chin J Biotechnol, 2006, 22(5): 744-749.
[10] Fladung N, Nowitzki O, Kumar S, Hoenicka H. The site-specific recombination systems Cre-lox and FLP-FRT are functionally active in poplar. For Genet, 2005, 12(2): 121-130.
[11] Fladung M, Becker D. Targeted integration and removal of transgenes in hybrid aspen (Populus tremula L. ×P. tremuloides Michx.) using site-specific recombination systems. Plant Biol (Stuttg), 2010, 12(2): 334-340.
[12] Fladung M, Schenk TMH, Polak O, Becker D. Elimination of marker genes and targeted integration via FLP⁄FRT recombination system from yeast in hybrid aspen (Populus tremula L. ×P. tremuloides Michx.). Tree Genes Genomes, 2010, 6(2): 205-217.
[13] Yoder JI, Goldsbrough AP. Transformation systems for generating marker-free transgenic plants. Nat Biotechnol, 1994, 12(3): 263-267.
[14] Goldsbrough AP, Lastrella CN, Yoder JI. Transposition mediated re-positioningan subsequent eiimination of marker genes from transgenic tomato. Nat Biotechnol, 1993, 11(11): 1286-1292.
[15] Zubko E, Seutt C, Meyer P. Intrachromosomal recombination between attP regions as a tool to remove selectable marker genes from tobacco transgenes. Nat Biotechnol, 2000, 18(4): 442-445.
[16] Fu XD, Duc LT, Fontana S, Bong BB, Tinjuangjun P, Sud-hakar D, Twyman RM, Christou P, Kohli A. Linear transgene constructs lacking vector backbone sequences generate low-copy-number transgenic plants with simple integration patterns. Transgenic Res, 2000, 9(1): 11-19.
[17] 姚琴, 丛玲, 汪越胜, 陈明洁, 杨广笑, 何光源. 无载体框架序列转基因小麦中外源基因表达框的遗传分析. 遗传, 2006, 28(6): 695-698.
[18] Romano A, Raemakers K, Bernardi J, Visser R, Mooibroek H. Transgene organisation in potato after particle bombardment-mediated (co-)transformation using plasmids and gene cassettes. Transgenic Res, 2003, 12(4): 461-473.
[19] Gao XR, Wang GK, Su Q, Wang Y, An LJ. Phytase expression in transgenic soybeans: stable transformation with a vector-less construct. Biotechnol Lett, 2007, 29(11): 1781-1787.
[20] Wu W, Su Q, Xia XY, Wang Y, Luan YS, An LJ. The Suaeda liaotungensis kitag betaine aldehyde dehy-drogenase gene improves salt tolerance of transgenic maize mediated with minimum linear length of DNA fragment. Euphytica, 2008, 159(1-2): 17-25.
[21] Hohn B,

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安全转基因技术研究进展

Advances on transgene containment technologies

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