遗传 ›› 2011, Vol. 33 ›› Issue (5): 449-458.doi: 10.3724/SP.J.1005.2011.00449

• 综述 • 上一篇    下一篇

转基因动物新技术研究进展

罗庆苗, 苗向阳, 张瑞杰   

  1. 中国农业科学院北京畜牧兽医研究所, 北京 100193
  • 收稿日期:2010-11-18 修回日期:2011-03-16 出版日期:2011-05-20 发布日期:2011-05-25
  • 通讯作者: 苗向阳 E-mail:mxy32@sohu.com
  • 基金资助:

    转基因生物新品种培育科技重大专项(编号:2009ZX08008-004B, 2008ZX08008-003), 国家高技术研究发展计划(863计划)项目 (编号:2008AA10Z140), 国家自然科学基金项目(编号:30571339)和中国农业科学院创新基金项目(编号:2004-院-1)资助

An update on the development of transgenic animal technology

LUO Qing-Miao, MIAO Xiang-Yang, ZHANG Rui-Jie   

  1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2010-11-18 Revised:2011-03-16 Online:2011-05-20 Published:2011-05-25
  • Contact: MIAO Xiang-Yang E-mail:mxy32@sohu.com

摘要: 转基因技术经过数十年的发展日渐成熟, 并推动转基因技术研究进入一个新的发展阶段。文章综述了体细胞核移植技术、慢病毒载体法、转座子介导的基因转移法、RNA干扰介导的基因敲除法和锌指核酸酶-基因打靶技术等近年发展起来的方法。而近来诱导多能干细胞(iPS 细胞)的成功为尚未获得ES细胞的大动物建立多能干细胞系提供了一种新的方案, 为转基因动物研究开创一片更广阔的天地。文章在前人研究的基础上重点总结了以上各种转基因技术的最新研究动态并对各种转基因技术的特点进行了探讨。

关键词: 转基因技术, RNAi, 基因打靶, 锌指核酸酶, iPS, 动物克隆

Abstract: The animal transgenic technology has increasingly turned mature over several decades and promoted the research of transgenic technology to a new developmental phase. In this review, various kinds of transgenic technologies, including somatic cell nuclear transfer, gene transfer mediated by transposon, gene knockout mediated by RNA interference, and zinc-finger nucleases-gene targeting technology, are summarized. Recently, the success of induced pluripotent stem cells (iPS cells), which has provided an alternative way to derive pluripotent stem cells of large animals, will extend the field of transgenic animal studies. Here, we summarized the latest trends on the basis of previous studies. In addition, the characteristics of different kinds of transgenic methods in detail are discussed.

Key words: transgenic technology, RNA interference, gene targeting, zinc-finger nuclease, iPS, animal cloning