家蚕核型多角体病毒水平转移基因分析

doi:10.3724/SP.J.1005.2011.00636

遗传 ›› 2011, Vol. 33 ›› Issue (6): 636-647.doi: 10.3724/SP.J.1005.2011.00636

家蚕核型多角体病毒水平转移基因分析

段海蓉^{1, 3}, 丘德彬², 贡成良³, 黄茉莉¹

1. 苏州大学系统生物学研究中心, 苏州 215006 2. 苏州大学数学科学学院, 苏州 215006 3. 苏州大学医学部, 苏州 215123

收稿日期:2010-09-29 修回日期:2010-12-14 出版日期:2011-06-20 发布日期:2011-06-25
通讯作者: 贡成良 E-mail:gongcl@suda.edu.cn
基金资助:
国家重点基础研究发展计划“973”项目(编号：2005CB121000), 江苏省高校自然科学研究项目(编号：SZ107917)和2009年度大学生创新性实验计划项目(编号：57315969)资助

Analysis of horizontal transfer gene of Bombyx mori NPV

DUAN Hai-Rong^{1, 3}, QIU De-Bin², GONG Cheng-Liang³, HUANG Mo-Li¹

1. Center for Systems Biology of Soochow University, Suzhou 215006, China 2. School of Mathematical Sciences of Soochow University, Suzhou 215006, China 3. Medical College of Soochow University, Suzhou 215123, China

Received:2010-09-29 Revised:2010-12-14 Online:2011-06-20 Published:2011-06-25
Contact: GONG Cheng-Liang E-mail:gongcl@suda.edu.cn

摘要/Abstract

摘要： 为了探讨杆状病毒基因组的遗传进化模式, 文章利用家蚕核型多角体病毒(BmNPV)和其宿主家蚕全基因组数据, 进行了全基因组的同源性搜索和系统进化分析, 结果显示, BmNPV的几丁质酶(Chi)基因、凋亡抑制蛋白3(IAP3)基因和尿苷二磷酸葡萄糖转移酶(UGT)基因为水平转移基因。这3个基因都来源于其宿主昆虫。通过核苷酸组成、密码子偏好性、选择压力等基因特征分析, 发现BmNPV水平转移基因与其基因组序列存在明显差异, 进一步验证水平转移基因的外源性。对3个水平转移基因的功能分析发现它们有利于杆状病毒在宿主昆虫中的侵染与繁殖, 并提高杆状病毒在昆虫中的生存能力

关键词: 家蚕核型多角体病毒, 家蚕, 杆状病毒, 水平转移基因

Abstract: For research on genetic characters and evolutionary origin of the genome of baculoviruses, a comprehensive homology search and phylogenetic analysis of the complete genomes of Bombyx mori NPV and Bombyx mori were used. Three horizontally transferred genes (inhibitor of apoptosis, chitinase, and UDP-glucosyltransferase) were identified, and there was evidence that all of these genes were derived from the insect host. The results of analysis showed lots of differences between the features of horizontal transferred genes and the ones of whole genomic genes, such as nucleotide composition, codon usagebias and selection pressure. These results reconfirmed that the horizontally transferred genes are exogenous. The analysis of gene function suggested that horizontally transferred genes acquired from an ancestral host in-sect can increase the efficiency of baculoviruses transmission.

Key words: BmNPV, Bombyx mori, baculoviruses, horizontal gene transfer

段海蓉，丘德彬，贡成良，黄茉莉. 家蚕核型多角体病毒水平转移基因分析[J]. 遗传, 2011, 33(6): 636-647.

DUAN Hai-Rong, QIU De-Ban, GONG Cheng-Liang, HUANG Mo-Chi. Analysis of horizontal transfer gene of Bombyx mori NPV[J]. HEREDITAS, 2011, 33(6): 636-647.

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家蚕核型多角体病毒水平转移基因分析

Analysis of horizontal transfer gene of Bombyx mori NPV

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