结合SSR标记和STS标记对家蚕无鳞毛翅基因的定位

doi:10.3724/SP.J.1005.2010.00054

遗传 ›› 2010, Vol. 32 ›› Issue (1): 54-58.doi: 10.3724/SP.J.1005.2010.00054

结合SSR标记和STS标记对家蚕无鳞毛翅基因的定位

王修业¹, 李木旺^{1, 2}, 赵云坡³, 徐安英^{1, 2}, 郭秋红³, 黄勇平³, 郭锡杰^{1, 2}

1. 中国农业科学院蚕业研究所, 镇江212018; 2. 江苏科技大学生物技术与环境工程学院, 镇江212018; 3. 中国科学院上海植物生理生态研究所, 上海200032

收稿日期:2009-05-26 修回日期:2009-09-13 出版日期:2010-01-20 发布日期:2010-01-15
通讯作者: 李木旺 E-mail:muwang_li@hotmail.com
基金资助:
国家重点基础研究发展计划(973计划)项目(编号：2005CB121004), 农业部行业专项(编号：nyhyzx07-020)和江苏省青蓝工程项目资助

Mapping of non-lepis wing gene nlw in silkworm (Bombyx mori) using SSR and STS markers

WANG Xiu-Ye¹, LI Mu-Wang^{1, 2}, ZHAO Yun-Po³, XU An-Ying^{1, 2}, GUO Qiu-Hong³, HUANG Yong-Ping³,
GUO Xi-Jie^{1, 2}

1. Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China; 2. College of Biotechnology and Environmental Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, China; 3. Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China

Received:2009-05-26 Revised:2009-09-13 Online:2010-01-20 Published:2010-01-15
Contact: LI Mu-Wang E-mail:muwang_li@hotmail.com

摘要/Abstract

摘要：

家蚕突变表型无鳞毛翅(non-lepis wing, nlw)由隐性基因nlw控制。由于家蚕雌性不发生交换, 文章采用有鳞毛翅品系P50和无鳞毛翅品系U06两个品系组配F1代及BC₁回交群体, (U06×P50)×U06和U06×(U06×P50)分别记作BC₁F和BC₁M, 根据已经构建的家蚕SSR分子标记连锁图谱及已经发表的有关序列对nlw基因进行了连锁及定位分析。得到8个与nlw基因连锁的SSR(Simple sequence repeat)标记和1个STS(Sequence-tagged sites)标记。BC₁F群中的所有正常翅个体均表现出与(U06×P50)F₁相同的杂合带型; 而所有无鳞毛个体带型与亲本U06一致, 为纯合型。利用BC₁M群体构建了关于nlw基因的遗传连锁图, 连锁图的遗传距离为125.7 cM, 与nlw基因最近的引物为STS标记cash2p, 图距为11.4 cM。

关键词: 家蚕, 无鳞毛基因, 基因定位, SSR, STS

Abstract:

The non-lepis wing of silkworm (Bombyx mori) is controlled by the recessive gene, nlw. Owning to lack of crossing over in females, the reciprocal backcrossed F₁ (BC₁) progenies were used for linkage analysis and mapping of nlw based on the SSR linkage map and STS markers using the wild type (+^nlw/+^nlw) silkworm strain P50 and U06 with scaleless wing (nlw/nlw). The nlw gene was linked to eight SSR markers and one STS marker. All the individuals with the wild type in the BC₁F (Using F₁ as female to backcross to the recessive parent, that is (U06×P50)×U06) showed heterozygous profile of (U06×P50) F₁, and the ones with non-lepis wing in BC₁F exhibited the homozygous profile of the strain U06. Using a reciprocal BC₁M (Using F₁ as male to backcross to the recessive parent, that is U06×(U06×P50))cross, we constructed a linkage map of 125.6 cM, and the distance between nlw and the nearest marker cash2p was 11.4 cM.

Key words: Bombyx mori, non-lepis wing, Gene linkage map, SSR, STS

王修业，李木旺，赵云坡，徐安英，郭秋红，黄勇平，郭锡杰. 结合SSR标记和STS标记对家蚕无鳞毛翅基因的定位[J]. 遗传, 2010, 32(1): 54-58.

WANG Xiu-Ye, LI Mu-Wang, DIAO Yun-Po, XU An-Yang, GUO Qiu-Gong, HUANG Yong-Beng, GUO Ti-Jie. Mapping of non-lepis wing gene nlw in silkworm (Bombyx mori) using SSR and STS markers[J]. HEREDITAS, 2010, 32(1): 54-58.

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结合SSR标记和STS标记对家蚕无鳞毛翅基因的定位

Mapping of non-lepis wing gene nlw in silkworm (Bombyx mori) using SSR and STS markers

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