遗传 ›› 2012, Vol. 34 ›› Issue (3): 366-370.doi: 10.3724/SP.J.1005.2012.00366

• 研究报告 • 上一篇    下一篇

番茄黄化曲叶病毒的快速分子检测

李常保1, 崔彦玲1, 张丽英1, 李传友2   

  1. 1. 北京市农林科学院蔬菜研究中心, 北京100097 2. 中国科学院遗传与发育生物学研究所, 北京100101
  • 收稿日期:2011-10-05 修回日期:2011-11-04 出版日期:2012-03-20 发布日期:2012-03-25
  • 通讯作者: 李常保 E-mail:lichangbao@nercv.org
  • 基金资助:

    国家公益性行业科研专项(编号:201003065), 国家高技术研究发展计划项目(863计划)(编号:2009AA10Z104), 北京市优秀人才项目、北京主要作物高效育种关键技术的合作研发项目和蔬菜高效育种技术研发与重大品种选育项目(编号:D11110000131102)(编号:2010DFB33740)资助

Molecular detection of tomato yellow leaf curl virus (TYLCV)

LI Chang-Bao1, CUI Yan-Ling1, ZHANG Li-Ying1, LI Chuan-You2   

  1. 1. Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China 2. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
  • Received:2011-10-05 Revised:2011-11-04 Online:2012-03-20 Published:2012-03-25

摘要: 番茄黄化曲叶病毒是当前世界范围内危害番茄生产的毁灭性病害。文章针对番茄黄化曲叶病毒全基因组序列的特异区段自主设计了1对特异性PCR引物(上游引物TYLCV-F:5′-ACGCATGCCTCTAATCCAGTGTA-3′, 下游引物TYLCV-R:5′-CCAATAAGGCGTAAGCGTGTAGAC-3′), 依据PCR扩增特异片段543 bp的有无可以快速、准确、高效、特异地检测出是否感染了TYLCV病毒, 这项技术可以方便地应用到工厂化育苗的带毒性检测、蔬菜大规模生产中植株发病情况的快速检测以及抗病毒育种, 从而为蔬菜安全可持续生产提供科技支撑。

关键词: 特异引物, 分子检测, 番茄黄化曲叶病毒

Abstract: Tomato yellow leaf curl virus (TYLCV) is currently considered as one of the most devastating viruses in cultivated tomatoes (Solanum lycopersicum) worldwide. We reported here the development of a PCR-based method to quickly detect TYLCV using the primer pairs (TYLCV-F: 5'-ACG CAT GCC TCT AAT CCA GTG TA-3' and TYLCV-R: 5'-CCA ATA AGG CGT AAG CGT GTA GAC-3'), which was designed based on the genome sequence of TYLCV. A TYLCV-specific band of 543 bp was amplified from infected tomato plants. This protocol provides a rapid, reliable, and sensitive tool for molecular detection and identification of TYLCV in the industrial seedling and virus resistance breeding to facilitate safe and sustainable production of tomato.

Key words: tomato yellow leaf curl virus (TYLCV), specific primer, molecular detection