遗传 ›› 2006, Vol. 28 ›› Issue (11): 1371-1375.doi: 10.1360/yc-006-1371

• 研究报告 • 上一篇    下一篇

秦川牛和中国荷斯坦牛POU1F1基因多态性研究

严林俊1①; 刘 波2①; 房兴堂1; 陈 宏1, 2; 张润锋2; 鲍 斌1; 张海军1   

  1. 1. 徐州师范大学细胞与分子生物学研究所,徐州 221116; 2. 西北农林科技大学动物科技学院
    陕西省农业分子生物学重点实验室, 杨凌712100

  • 收稿日期:2005-12-30 修回日期:2006-02-27 出版日期:2006-11-05 发布日期:2006-11-05
  • 通讯作者: 陈 宏

Analysis of POU1F1 Gene Polymorphisms in Qinchuan Cattle and Chinese Holstein Cattle

YAN Lin-Jun1; LIU Bo2; FANG Xin-Tang1; CHEN Hong1,2; ZHANG Run-Feng2;
BAO Bin1; ZHANG Hai-Jun1
  

  1. 1. Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou 221116, China; 2. College of Animal Science
    and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China
  • Received:2005-12-30 Revised:2006-02-27 Online:2006-11-05 Published:2006-11-05
  • Contact: CHEN Hong

PDF (PC)

2351

被引次数

33

摘要：

采用PCR-RFLP技术研究了秦川牛(QQ)和中国荷斯坦牛(HC)共计218头个体POU1F1基因的多态性。结果表明: 秦川牛及中国荷斯坦牛群体POU1F1-HinfⅠ基因座的451 bp 的PCR产物经限制性酶HinfⅠ消化后表现多态, 其等位基因A/B频率分别为0.232/0.768、0.132/0.868; 两个群体AA、AB和BB 3种基因型的频率分别为0.030/0.403/0.567、0.007/0.251/0.742。在该基因座秦川牛群体处于Hardy-Weinberg平衡状态, 中国荷斯坦牛群体处于不平衡状态。它们在该基因座的杂合度、有效等位基因数、Shannon信息熵、多态信息含量分别为0.356/1.553/0.541/0.292、0.229/1.297/0.390/0.203; 秦川牛群体的位点杂合度、有效等位基因数、Shannon信息熵、多态信息含量均大于中国荷斯坦牛群体。

关键词: POU1F1, 秦川牛, 多态性, PCR-RFLP, 荷斯坦牛

Abstract:

PCR-RFLP was applied to analyze the polymorphisms of POU1F1 gene in 218 Qinchuan cattle (QQ) and Chinese Holstein cattle (HC). Results demonstrated HinfⅠ polymorphisms in the 451 bp PCR product in the two populations. The frequencies of alleles A/B in QQ and HC populations were 0.232/0.768 and 0.132/0.868, respectively. The frequencies of three genotypes AA, AB and BB were 0.030/0.403/0.567 and 0.007/0.251/0.742, respectively. Qinchuan cattle population was at Hardy-Weinberg equilibrium at this locus, but Chinese Holstein cattle population was not. The gene heterozygosity/effective allele gene number/Shannon information entropy /polymorphism information content of Qinchuan cattle and Chinese Holstein cattle populations were listed for 0.356/1.553/0.541/0.292 and 0.229/1.297/0.390/0.203, respectively. All indices were higher in the Qinchuan cattle population.

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