遗传 ›› 2007, Vol. 29 ›› Issue (4): 475-482.doi: 10.1360/yc-007-0475

• 研究报告 • 上一篇    下一篇

甘蓝型油菜理化诱变和突变体库的构建

孙加焱, 涂进东, 范叔味, 吴建国, 石春海   

  1. 浙江大学农业与生物技术学院农学系, 杭州 310029

  • 收稿日期:2006-05-30 修回日期:2007-02-02 出版日期:2007-04-10 发布日期:2007-04-10
  • 通讯作者: 石春海,吴建国

The screening of mutants induced by physical and chemical factors and construction of mutant population for Brassica napus L.

SUN Jia-Yan, TU Jin-Dong, FAN Shu-Wei, WU Jian-Guo, SHI Chun-Hai

  

  1. Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China
  • Received:2006-05-30 Revised:2007-02-02 Online:2007-04-10 Published:2007-04-10
  • Contact: SHI Chun-Hai ,WU Jian-Guo

摘要:

利用射线与甲基磺酸乙酯(EMS)溶液诱变技术复合处理甘蓝型油菜高油605的成熟种子。经田间M2筛选和M3验证, 共筛选到152个叶色、叶形、株高、分枝数、分枝角度、茎径、茎色、花色、花瓣数、花瓣大小、花蕊形态、雄性不育、死蕾及开花期等性状发生变异的突变体, 占M2诱变群体总数的12.67%。利用水培法进行M3和M4株系的子叶和根系变异性状筛选和验证, 发现子叶和根系变异频率分别为12.78%和7.07%。现已构建了包括叶片、株型、花器、子叶、根系及部分生理性状变异类型的突变体库, 可为今后油菜遗传改良和功能基因组学研究提供更多的种质资源。

关键词: 油菜, 诱变, , 射线,

Abstract:

The mature seeds of rapeseed (Brassica napus L.) from variety Gaoyou 605 were treated with -rays and Ethyl Methan Sulfonate (EMS). 152 mutants (12.67% of M2 population) with mutative traits, including the mutation of leaf color, leaf shape, plant height, number and angle of branches, diameter of main stalk, color of stalk and flower, number and size of petals, pistil shape, male sterility, bud death and date of bloom were found in screened M2 progenies, which have been identified in M3. The mutants of cotyledon and root traits were also screened by hydroponics culture and their total mutant frequency were estimated at 12.78% and 7.07% in M3, respectively. Identification of M4 showed that these mutations could be inherited stably. The mutant library including the mutants of leaf, plant-type, flower, cotyledon, root and physiological traits had been built in present experiment. These mutants might be used as important germplasm for rapeseed breeding and functional genomics study.