遗传 ›› 2008, Vol. 30 ›› Issue (7): 863-869.doi: 10.3724/SP.J.1005.2008.00863

• 研究报告 • 上一篇    下一篇

牛凝乳酶原基因克隆及序列的进化分析

孙大庆1; 姜毓君1, 2; 韩希妍1; 曲妍妍1; 毕宇涵1; 张光辉1   

  1. 1. 东北农业大学 乳品科学教育部重点实验室, 哈尔滨 150030;
    2. 国家乳业工程技术研究中心, 哈尔滨 150086

  • 收稿日期:2007-11-30 修回日期:2007-01-09 出版日期:2008-07-10 发布日期:2008-07-10
  • 通讯作者: 张光辉

Cloning and phylogenetic analysis of bovine prochymosin gene

SUN Da-Qing1; JIANG Yu-Jun1, 2; HAN Xi-Yan1; QU Yan-Yan1; BI Yu-Han1;
ZHANG Guang-Hui1

  

  1. 1. Key Lab of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China; 2. National Research Center of Dairy Engineering and Technology, Harbin 150086, China
  • Received:2007-11-30 Revised:2007-01-09 Online:2008-07-10 Published:2008-07-10
  • Contact: ZHANG Guang-Hui

摘要:

利用RT-PCR克隆获得牛凝乳酶原基因的cDNA序列, 测序后与GenBank中凝乳酶原基因进行序列比对和生物信息学分析。序列比对统计分析显示, 该基因为牛凝乳酶原B基因, 与已知牛和其他哺乳动物的凝乳酶原基因具有很高的同源性,18种哺乳动物凝乳酶原基因密码子一、二、三位点的碱基偏倚度分别为:6.227、1.042和1.456。这表明该基因具有整体保守性和突变位点偏倚性两个特征, 可以作为哺乳动物系统进化的研究对象。采用多种方法构建的该基因系统进化树一致表明, 偶蹄动物与灵长动物的亲缘关系比偶蹄动物与啮齿动物的亲缘关系更近, 比偶蹄动物与食肉动物的亲缘关系更远, 并且18种哺乳动物的亲缘关系与动物种系进化关系一致, 为哺乳动物系统进化关系研究提供了分子水平的佐证和依据。

关键词: 凝乳酶原, 序列比对, 同源性, 系统进化

Abstract:

The cDNA sequence of bovine prochymosin gene was cloned and sequenced from the abomasums of suckling calf by RT-PCR. The sequence was aligned and bioinformatically analyzed with related sequences in GenBank. The result of sequence analysis revealed that the gene was determined to bovine prochymosin B gene and had the high level of homology with prochymosin gene of other known mammals. The base bias of 18 species of prochymosin gene reduced according to codon position, and the gene provided us with excellent material ofphylogenetic research. Thus, the phylogenetic tree of 18 species of prochymosin gene was used to discuss and offer testimony to phylogenetic relationship of 11 mammals.